The domain of brain β-spectrin responsible for synaptic vesicle association is essential for synaptic transmission

Warren E. Zimmer, Ying Zhao, Aleksander F. Sikorski, Stuart D. Critz, José Sangerman, Lisa A. Elferink, X. Susan Xu, Steven Goodman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have examined the interaction between synapsin I, the major phosphoprotein on the membrane of small synaptic vesicles, and brain spectrin. Using recombinant peptides we have localized the synapsin I attachment site upon the β-spectrin isoform βSpIIΣI to a region of 25 amino acids, residues 211 through 235. This segment is adjacent to the actin binding domain and is within the region of the βSpIIΣI that we previously predicted as a candidate synapsin I binding domain based upon sequence homology. We used differential centrifugation techniques to quantitatively assess the interaction of spectrin with synaptic vesicles. Using this assay, high affinity saturable binding of recombinant βSpIIΣI proteins was observed with synaptic vesicles. Binding was only observed when the 25 amino acid synapsin I binding site was included on the recombinant peptides. Further, we demonstrate that antibodies directed against 15 amino acids of the synapsin I binding domain specifically blocked synaptic transmission in cultured hippocampal neurons. Thus, the synapsin I attachment site on βSpIIΣI spectrin comprises a ~25 amino acid segment of the molecule and interaction of these two proteins is an essential step for the process of neurotransmission. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)18-27
Number of pages10
JournalBrain Research
Volume881
Issue number1
DOIs
StatePublished - Oct 20 2000
Externally publishedYes

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Synapsins
Spectrin
Synaptic Vesicles
Synaptic Transmission
Brain
Amino Acids
Peptides
Phosphoproteins
Sequence Homology
Centrifugation
Recombinant Proteins
Actins
Protein Isoforms
Binding Sites
Neurons
Membranes
Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

The domain of brain β-spectrin responsible for synaptic vesicle association is essential for synaptic transmission. / Zimmer, Warren E.; Zhao, Ying; Sikorski, Aleksander F.; Critz, Stuart D.; Sangerman, José; Elferink, Lisa A.; Xu, X. Susan; Goodman, Steven.

In: Brain Research, Vol. 881, No. 1, 20.10.2000, p. 18-27.

Research output: Contribution to journalArticle

Zimmer, Warren E. ; Zhao, Ying ; Sikorski, Aleksander F. ; Critz, Stuart D. ; Sangerman, José ; Elferink, Lisa A. ; Xu, X. Susan ; Goodman, Steven. / The domain of brain β-spectrin responsible for synaptic vesicle association is essential for synaptic transmission. In: Brain Research. 2000 ; Vol. 881, No. 1. pp. 18-27.
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