The E3 ligase TRIM56 is a host restriction factor of zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function

Darong Yang, Nan L. Li, Dahai Wei, Baoming Liu, Fang Guo, Husni Elbahesh, Yunzhi Zhang, Zhi Zhou, Guo Yun Chen, Kui Li

Research output: Contribution to journalArticle

Abstract

Infection by Zika virus (ZIKV) is linked to microcephaly and other neurological disorders, posing a significant health threat. Innate immunity is the first line of defense against invading pathogens, but relatively little is understood regarding host intrinsic mechanisms that guard against ZIKV. Here, we show that host tripartite motif-containing protein 56 (TRIM56) poses a barrier to ZIKV infection in cells of neural, epithelial and fibroblast origins. Overexpression of TRIM56, but not an E3 ligase-dead mutant or one lacking a short C-terminal portion, inhibited ZIKV RNA replication. Conversely, depletion of TRIM56 increased viral RNA levels. Although the C-terminal region of TRIM56 bears sequence homology to NHL repeat of TRIM-NHL proteins that regulate miRNA activity, knockout of Dicer, which abolishes production of miRNAs, had no demonstrable effect on ZIKV restriction imposed by TRIM56. Rather, we found that TRIM56 is an RNA-binding protein that associates with ZIKV RNA in infected cells. Moreover, a recombinant TRIM56 fragment comprising the C-terminal 392 residues captured ZIKV RNA in cell-free reactions, indicative of direct interaction. Remarkably, deletion of a short C-terminal tail portion abrogated the TRIM56-ZIKV RNA interaction, concomitant with a loss in antiviral activity. Altogether, our study reveals TRIM56 is an RNA binding protein that acts as a ZIKV restriction factor and provides new insights into the antiviral mechanism by which this E3 ligase tackles flavivirus infections.

Original languageEnglish (US)
Article numbere0007537
JournalPLoS neglected tropical diseases
Volume13
Issue number6
DOIs
StatePublished - Jun 1 2019

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Ubiquitin-Protein Ligases
MicroRNAs
Antiviral Agents
RNA
RNA-Binding Proteins
Flavivirus Infections
Zika Virus
Tripartite Motif Proteins
Microcephaly
Viral RNA
Virus Replication
Sequence Homology
Nervous System Diseases
Recombinant Proteins
Innate Immunity
Fibroblasts
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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The E3 ligase TRIM56 is a host restriction factor of zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function. / Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo Yun; Li, Kui.

In: PLoS neglected tropical diseases, Vol. 13, No. 6, e0007537, 01.06.2019.

Research output: Contribution to journalArticle

Yang, Darong ; Li, Nan L. ; Wei, Dahai ; Liu, Baoming ; Guo, Fang ; Elbahesh, Husni ; Zhang, Yunzhi ; Zhou, Zhi ; Chen, Guo Yun ; Li, Kui. / The E3 ligase TRIM56 is a host restriction factor of zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function. In: PLoS neglected tropical diseases. 2019 ; Vol. 13, No. 6.
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