The effect of α-adrenergic antagonism upon nitrogen loss during endotoxemia

Roland Dickerson, David A. Kuhl, Rex Brown, J. Travis Methvin, Jay F. Mouser, B. Hak Emily, Lawrence J. Hak

Research output: Contribution to journalArticle

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Abstract

Sixty male Sprague-Dawley rats were randomized to receive parenteral nutrition (PN) only; PN plus continuous infusion of Escherichia coli 026:B6 lipopolysaccharide (PN + LPS) at 6 mg · kg-1 · d-1; or PN plus LPS plus a continuous infusion of the α-adrenergic antagonist phentolamine (PN + LPS + PHEN) at 5 mg · kg-1 · d-1 or 20 mg · kg-1 · d-1 for 48 h. All animals received isocaloric, isonitrogenous PN. LPS significantly lowered nitrogen balance (mmol/48 h) from PN control; however, addition of PHEN substantially worsened nitrogen balance compared with LPS (14.2 ± 3, 2.4 ± 5.2, -1.6 ± 4.5, -0.8 ± 5.4, for the PN, PN + LPS, PN + LPS + PHEN5 and PN + LPS + PHEN20 groups, respectively; P < 0.0001). Urinary 3- methylhistidine/creatinine ratio (3-meH/creat) paralleled the nitrogen balance data (0.30 ± 0.09, 0.45 ± 0.12, 0.51 ± 0.14, 0.60 ± 0.12, respectively, P < 0.0001). The high-dose PHEN resulted in 82 ± 9% blockade. To ascertain if any beneficial effect upon body protein loss is achieved during severe stress, 30 rats were given PN+LPS at 12 mg · kg-1 · d-1 or PN+LPS12+PHEN20. These data showed similar changes in nitrogen balance and 3-methylhistidine/creatinine with the use of PHEN during severe endotoxemia, α-adrenergic antagonism with PHEN worsens body protein loss as measured by nitrogen balance and 3-methylhistadine/creatinine in PN-fed endotoxemic rats.

Original languageEnglish (US)
Pages (from-to)887-894
Number of pages8
JournalNutrition
Volume13
Issue number10
DOIs
StatePublished - Oct 1 1997

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Endotoxemia
Parenteral Nutrition
Adrenergic Agents
Nitrogen
Creatinine
Adrenergic Antagonists
Phentolamine
Sprague Dawley Rats
Lipopolysaccharides

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Dickerson, R., Kuhl, D. A., Brown, R., Methvin, J. T., Mouser, J. F., Emily, B. H., & Hak, L. J. (1997). The effect of α-adrenergic antagonism upon nitrogen loss during endotoxemia. Nutrition, 13(10), 887-894. https://doi.org/10.1016/S0899-9007(97)00267-0

The effect of α-adrenergic antagonism upon nitrogen loss during endotoxemia. / Dickerson, Roland; Kuhl, David A.; Brown, Rex; Methvin, J. Travis; Mouser, Jay F.; Emily, B. Hak; Hak, Lawrence J.

In: Nutrition, Vol. 13, No. 10, 01.10.1997, p. 887-894.

Research output: Contribution to journalArticle

Dickerson, R, Kuhl, DA, Brown, R, Methvin, JT, Mouser, JF, Emily, BH & Hak, LJ 1997, 'The effect of α-adrenergic antagonism upon nitrogen loss during endotoxemia', Nutrition, vol. 13, no. 10, pp. 887-894. https://doi.org/10.1016/S0899-9007(97)00267-0
Dickerson, Roland ; Kuhl, David A. ; Brown, Rex ; Methvin, J. Travis ; Mouser, Jay F. ; Emily, B. Hak ; Hak, Lawrence J. / The effect of α-adrenergic antagonism upon nitrogen loss during endotoxemia. In: Nutrition. 1997 ; Vol. 13, No. 10. pp. 887-894.
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AU - Emily, B. Hak

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N2 - Sixty male Sprague-Dawley rats were randomized to receive parenteral nutrition (PN) only; PN plus continuous infusion of Escherichia coli 026:B6 lipopolysaccharide (PN + LPS) at 6 mg · kg-1 · d-1; or PN plus LPS plus a continuous infusion of the α-adrenergic antagonist phentolamine (PN + LPS + PHEN) at 5 mg · kg-1 · d-1 or 20 mg · kg-1 · d-1 for 48 h. All animals received isocaloric, isonitrogenous PN. LPS significantly lowered nitrogen balance (mmol/48 h) from PN control; however, addition of PHEN substantially worsened nitrogen balance compared with LPS (14.2 ± 3, 2.4 ± 5.2, -1.6 ± 4.5, -0.8 ± 5.4, for the PN, PN + LPS, PN + LPS + PHEN5 and PN + LPS + PHEN20 groups, respectively; P < 0.0001). Urinary 3- methylhistidine/creatinine ratio (3-meH/creat) paralleled the nitrogen balance data (0.30 ± 0.09, 0.45 ± 0.12, 0.51 ± 0.14, 0.60 ± 0.12, respectively, P < 0.0001). The high-dose PHEN resulted in 82 ± 9% blockade. To ascertain if any beneficial effect upon body protein loss is achieved during severe stress, 30 rats were given PN+LPS at 12 mg · kg-1 · d-1 or PN+LPS12+PHEN20. These data showed similar changes in nitrogen balance and 3-methylhistidine/creatinine with the use of PHEN during severe endotoxemia, α-adrenergic antagonism with PHEN worsens body protein loss as measured by nitrogen balance and 3-methylhistadine/creatinine in PN-fed endotoxemic rats.

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