The effect of aging on bone formation in rats: Biochemical and histological evidence for decreased bone formation capacity

Satoru Nishimoto, Chung Ho Chang, Elchonon Gendler, William F. Stryker, Marcel E. Nimni

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Ectopic bone formation by subcutaneously implanted demineralized bone matrix powder (DBM) was assessed biochemically and histologically in Fischer 344 rats of different ages. The total calcium accumulated in implants was greatly depressed in older rats, as was the rate of45Ca deposition. High alkaline phosphatase activity appeared later in the 10- and 16-month-old rats compared with 1-month-old rats, and the magnitude of the alkaline phosphatase activity was decreased in 16-month-old rats. The accumulation of the bone-specific vitamin K-dependent bone protein (bone gla protein, BGP) was decreased in the implants in older rats. Histological examination of the implants confirms the decreased ability of aged animals to produce bone in response to DBM. Measurements of total calcium, alkaline phosphatase, and BGP at the site of demineralized bone matrix implants clearly demonstrates that bone formation decreases dramatically with increasing age. Significant differences in total calcium can be detected even between 1-month-old and 3-month-old rats. Serum BGP shows a marked decrease (47%) between 1-month- and 3-month-old rats, a decrease not paralleled by a similar decrease in BGP present in calvarial or tibial bone.

Original languageEnglish (US)
Pages (from-to)617-624
Number of pages8
JournalCalcified Tissue International
Volume37
Issue number6
DOIs
StatePublished - Nov 1 1985
Externally publishedYes

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Osteogenesis
Osteocalcin
Bone Matrix
Alkaline Phosphatase
Bone and Bones
Calcium
Powders
Inbred F344 Rats
Blood Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

The effect of aging on bone formation in rats : Biochemical and histological evidence for decreased bone formation capacity. / Nishimoto, Satoru; Chang, Chung Ho; Gendler, Elchonon; Stryker, William F.; Nimni, Marcel E.

In: Calcified Tissue International, Vol. 37, No. 6, 01.11.1985, p. 617-624.

Research output: Contribution to journalArticle

Nishimoto, Satoru ; Chang, Chung Ho ; Gendler, Elchonon ; Stryker, William F. ; Nimni, Marcel E. / The effect of aging on bone formation in rats : Biochemical and histological evidence for decreased bone formation capacity. In: Calcified Tissue International. 1985 ; Vol. 37, No. 6. pp. 617-624.
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