The effect of alcohol on the differential expression of cluster of differentiation 14 gene, associated pathways, and genetic network

Diana X. Zhou, Yinghong Zhao, Jessica A. Baker, Qingqing Gu, Kristin Hamre, Junming Yue, Byron Jones, Melloni N. Cook, Lu Lu

Research output: Contribution to journalArticle

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Abstract

Alcohol consumption affects human health in part by compromising the immune system. In this study, we examined the expression of the Cd14 (cluster of differentiation 14) gene, which is involved in the immune system through a proinflammatory cascade. Expression was evaluated in BXD mice treated with saline or acute 1.8 g/kg i.p. ethanol (12.5% v/v). Hippocampal gene expression data were generated to examine differential expression and to perform systems genetics analyses. The Cd14 gene expression showed significant changes among the BXD strains after ethanol treatment, and eQTL mapping revealed that Cd14 is a cis-regulated gene. We also identified eighteen ethanol-related phenotypes correlated with Cd14 expression related to either ethanol responses or ethanol consumption. Pathway analysis was performed to identify possible biological pathways involved in the response to ethanol and Cd14. We also constructed a genetic network for Cd14 using the top 20 correlated genes and present several genes possibly involved in Cd14 and ethanol responses based on differential gene expression. In conclusion, we found Cd14, along with several other genes and pathways, to be involved in ethanol responses in the hippocampus, such as increased susceptibility to lipopolysaccharides and neuroinflammation.

Original languageEnglish (US)
Article numbere0178689
JournalPLoS ONE
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2017

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Ethanol
alcohols
Genes
ethanol
Alcohols
genes
Gene expression
Immune system
Gene Expression
gene expression
immune system
Immune System
hippocampus
Alcohol Drinking
lipopolysaccharides
Lipopolysaccharides
human health
Hippocampus
Health
Phenotype

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The effect of alcohol on the differential expression of cluster of differentiation 14 gene, associated pathways, and genetic network. / Zhou, Diana X.; Zhao, Yinghong; Baker, Jessica A.; Gu, Qingqing; Hamre, Kristin; Yue, Junming; Jones, Byron; Cook, Melloni N.; Lu, Lu.

In: PLoS ONE, Vol. 12, No. 6, e0178689, 01.06.2017.

Research output: Contribution to journalArticle

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