The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse

Melissa J. Puppa, James P. White, Kandy T. Velázquez, Kristen A. Baltgalvis, Shuichi Sato, John W. Baynes, James Carson

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background Cachexia involves unintentional body weight loss including diminished muscle and adipose tissue mass and is associated with an underlying disease. Systemic overexpression of IL-6 accelerates cachexia in the ApcMin/+ mouse, but does not induce wasting in control C57BL/6 mice. With many chronic diseases, chronic inflammation and metabolic dysfunction can be improved with moderate exercise. A direct effect of regular moderate exercise on the prevention of IL-6-induced cachexia in the ApcMin/+ mouse has not been investigated. The purpose of this study was to assess the effects of exercise on the development of cachexia in the ApcMin/+ mouse. Methods Mice were randomly assigned to moderate treadmill exercise (18 m/min, 1 h, 6 days/week, 5% grade) or cage control (CC) groups from 6 to 14 weeks of age. At 12 weeks of age, mice were electroporated with either IL-6- containing or control plasmid into the quadriceps muscle. Mice were killed after 2 weeks of systemic IL-6 overexpression or control treatment. Results IL-6 overexpression induced an 8% loss in body weight in CC mice, which was significantly attenuated by exercise. IL-6 overexpression in CC mice increased fasting insulin and triglyceride levels, which were normalized by exercise, and associated with increased oxidative capacity, an induction of AKT signaling, and a repression of AMPK signaling in muscle. These exercise-induced changes occurred despite elevated inflammatory signaling in skeletal muscle. Conclusion We conclude that moderate-intensity exercise can attenuate IL-6-dependent cachexia in ApcMin/+ mice, independent of changes in IL-6 concentration and muscle inflammatory signaling. The exercise effect was associated with improved insulin sensitivity and improved energy status in the muscle.

Original languageEnglish (US)
Pages (from-to)117-137
Number of pages21
JournalJournal of Cachexia, Sarcopenia and Muscle
Volume3
Issue number2
DOIs
StatePublished - Jan 1 2012

Fingerprint

Cachexia
Interleukin-6
Muscles
Body Weight
AMP-Activated Protein Kinases
Quadriceps Muscle
Inbred C57BL Mouse
Insulin Resistance
Adipose Tissue
Weight Loss
Fasting
Skeletal Muscle
Triglycerides
Plasmids
Chronic Disease
Insulin
Inflammation
Control Groups

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine
  • Physiology (medical)

Cite this

Puppa, M. J., White, J. P., Velázquez, K. T., Baltgalvis, K. A., Sato, S., Baynes, J. W., & Carson, J. (2012). The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse. Journal of Cachexia, Sarcopenia and Muscle, 3(2), 117-137. https://doi.org/10.1007/s13539-011-0047-1

The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse. / Puppa, Melissa J.; White, James P.; Velázquez, Kandy T.; Baltgalvis, Kristen A.; Sato, Shuichi; Baynes, John W.; Carson, James.

In: Journal of Cachexia, Sarcopenia and Muscle, Vol. 3, No. 2, 01.01.2012, p. 117-137.

Research output: Contribution to journalArticle

Puppa, MJ, White, JP, Velázquez, KT, Baltgalvis, KA, Sato, S, Baynes, JW & Carson, J 2012, 'The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse', Journal of Cachexia, Sarcopenia and Muscle, vol. 3, no. 2, pp. 117-137. https://doi.org/10.1007/s13539-011-0047-1
Puppa MJ, White JP, Velázquez KT, Baltgalvis KA, Sato S, Baynes JW et al. The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse. Journal of Cachexia, Sarcopenia and Muscle. 2012 Jan 1;3(2):117-137. https://doi.org/10.1007/s13539-011-0047-1
Puppa, Melissa J. ; White, James P. ; Velázquez, Kandy T. ; Baltgalvis, Kristen A. ; Sato, Shuichi ; Baynes, John W. ; Carson, James. / The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse. In: Journal of Cachexia, Sarcopenia and Muscle. 2012 ; Vol. 3, No. 2. pp. 117-137.
@article{9478e4b1e1fe4574b8461c40b8f3ad12,
title = "The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse",
abstract = "Background Cachexia involves unintentional body weight loss including diminished muscle and adipose tissue mass and is associated with an underlying disease. Systemic overexpression of IL-6 accelerates cachexia in the ApcMin/+ mouse, but does not induce wasting in control C57BL/6 mice. With many chronic diseases, chronic inflammation and metabolic dysfunction can be improved with moderate exercise. A direct effect of regular moderate exercise on the prevention of IL-6-induced cachexia in the ApcMin/+ mouse has not been investigated. The purpose of this study was to assess the effects of exercise on the development of cachexia in the ApcMin/+ mouse. Methods Mice were randomly assigned to moderate treadmill exercise (18 m/min, 1 h, 6 days/week, 5{\%} grade) or cage control (CC) groups from 6 to 14 weeks of age. At 12 weeks of age, mice were electroporated with either IL-6- containing or control plasmid into the quadriceps muscle. Mice were killed after 2 weeks of systemic IL-6 overexpression or control treatment. Results IL-6 overexpression induced an 8{\%} loss in body weight in CC mice, which was significantly attenuated by exercise. IL-6 overexpression in CC mice increased fasting insulin and triglyceride levels, which were normalized by exercise, and associated with increased oxidative capacity, an induction of AKT signaling, and a repression of AMPK signaling in muscle. These exercise-induced changes occurred despite elevated inflammatory signaling in skeletal muscle. Conclusion We conclude that moderate-intensity exercise can attenuate IL-6-dependent cachexia in ApcMin/+ mice, independent of changes in IL-6 concentration and muscle inflammatory signaling. The exercise effect was associated with improved insulin sensitivity and improved energy status in the muscle.",
author = "Puppa, {Melissa J.} and White, {James P.} and Vel{\'a}zquez, {Kandy T.} and Baltgalvis, {Kristen A.} and Shuichi Sato and Baynes, {John W.} and James Carson",
year = "2012",
month = "1",
day = "1",
doi = "10.1007/s13539-011-0047-1",
language = "English (US)",
volume = "3",
pages = "117--137",
journal = "Journal of Cachexia, Sarcopenia and Muscle",
issn = "2190-5991",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - The effect of exercise on IL-6-induced cachexia in the ApcMin/+ mouse

AU - Puppa, Melissa J.

AU - White, James P.

AU - Velázquez, Kandy T.

AU - Baltgalvis, Kristen A.

AU - Sato, Shuichi

AU - Baynes, John W.

AU - Carson, James

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Background Cachexia involves unintentional body weight loss including diminished muscle and adipose tissue mass and is associated with an underlying disease. Systemic overexpression of IL-6 accelerates cachexia in the ApcMin/+ mouse, but does not induce wasting in control C57BL/6 mice. With many chronic diseases, chronic inflammation and metabolic dysfunction can be improved with moderate exercise. A direct effect of regular moderate exercise on the prevention of IL-6-induced cachexia in the ApcMin/+ mouse has not been investigated. The purpose of this study was to assess the effects of exercise on the development of cachexia in the ApcMin/+ mouse. Methods Mice were randomly assigned to moderate treadmill exercise (18 m/min, 1 h, 6 days/week, 5% grade) or cage control (CC) groups from 6 to 14 weeks of age. At 12 weeks of age, mice were electroporated with either IL-6- containing or control plasmid into the quadriceps muscle. Mice were killed after 2 weeks of systemic IL-6 overexpression or control treatment. Results IL-6 overexpression induced an 8% loss in body weight in CC mice, which was significantly attenuated by exercise. IL-6 overexpression in CC mice increased fasting insulin and triglyceride levels, which were normalized by exercise, and associated with increased oxidative capacity, an induction of AKT signaling, and a repression of AMPK signaling in muscle. These exercise-induced changes occurred despite elevated inflammatory signaling in skeletal muscle. Conclusion We conclude that moderate-intensity exercise can attenuate IL-6-dependent cachexia in ApcMin/+ mice, independent of changes in IL-6 concentration and muscle inflammatory signaling. The exercise effect was associated with improved insulin sensitivity and improved energy status in the muscle.

AB - Background Cachexia involves unintentional body weight loss including diminished muscle and adipose tissue mass and is associated with an underlying disease. Systemic overexpression of IL-6 accelerates cachexia in the ApcMin/+ mouse, but does not induce wasting in control C57BL/6 mice. With many chronic diseases, chronic inflammation and metabolic dysfunction can be improved with moderate exercise. A direct effect of regular moderate exercise on the prevention of IL-6-induced cachexia in the ApcMin/+ mouse has not been investigated. The purpose of this study was to assess the effects of exercise on the development of cachexia in the ApcMin/+ mouse. Methods Mice were randomly assigned to moderate treadmill exercise (18 m/min, 1 h, 6 days/week, 5% grade) or cage control (CC) groups from 6 to 14 weeks of age. At 12 weeks of age, mice were electroporated with either IL-6- containing or control plasmid into the quadriceps muscle. Mice were killed after 2 weeks of systemic IL-6 overexpression or control treatment. Results IL-6 overexpression induced an 8% loss in body weight in CC mice, which was significantly attenuated by exercise. IL-6 overexpression in CC mice increased fasting insulin and triglyceride levels, which were normalized by exercise, and associated with increased oxidative capacity, an induction of AKT signaling, and a repression of AMPK signaling in muscle. These exercise-induced changes occurred despite elevated inflammatory signaling in skeletal muscle. Conclusion We conclude that moderate-intensity exercise can attenuate IL-6-dependent cachexia in ApcMin/+ mice, independent of changes in IL-6 concentration and muscle inflammatory signaling. The exercise effect was associated with improved insulin sensitivity and improved energy status in the muscle.

UR - http://www.scopus.com/inward/record.url?scp=84866562969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866562969&partnerID=8YFLogxK

U2 - 10.1007/s13539-011-0047-1

DO - 10.1007/s13539-011-0047-1

M3 - Article

VL - 3

SP - 117

EP - 137

JO - Journal of Cachexia, Sarcopenia and Muscle

JF - Journal of Cachexia, Sarcopenia and Muscle

SN - 2190-5991

IS - 2

ER -