The effect of high cervical spinal cord stimulation on the expression of SP, NK-1 and TRPV1 mRNAs during cardiac ischemia in rat

Xiao Hui Ding, Deidra Mountain, Venkateswaran Subramanian, Krishna Singh, Carole Ann Williams

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Spinal cord stimulation (SCS) is used to reduce angina that accompanies cardiac ischemia, but little is known about the molecular mechanisms mediating this effect. We studied the expression of SP, neurokinin-1 (NK-1) receptor, and transient receptor potential vanilloid type 1 (TRPV1) mRNA in the rat spinal cord at thoracic 4 (T4), cervical 2 (C2) and caudal brain stem by RT-PCR during intermittent occlusion of the left anterior descending coronary artery (CoAO), during sustained SCS by itself at the C2 spinal segment, and during sustained SCS plus intermittent CoAO. Only SP mRNA was increased significantly in T4 and brainstem during CoAO, while SCS decreased the mRNA levels of SP, NK-1 and TRPV1 significantly in T4 and the brainstem. SCS attenuated the increase of SP and TRPV1 mRNA levels at T4 level induced by intermittent CoAO when the stimulation was applied prior to the initiation of the cardiac ischemia. These results support the role for SP as a putative neurotransmitter for the myocardial ischemia-sensitive afferent neuron signal to the spinal level. They suggest that modification of the ischemic cardiac nociceptive afferent signal by SCS involves a change in SP and TRPV1 expression.

Original languageEnglish (US)
Pages (from-to)139-144
Number of pages6
JournalNeuroscience Letters
Volume424
Issue number2
DOIs
StatePublished - Sep 7 2007
Externally publishedYes

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Spinal Cord Stimulation
Neurokinin-1 Receptors
Ischemia
Messenger RNA
Thorax
Brain Stem
Afferent Neurons
Myocardial Ischemia
Neurotransmitter Agents
Cervical Cord
Spinal Cord
Coronary Vessels
Polymerase Chain Reaction
vanilloid receptor subtype 1

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

The effect of high cervical spinal cord stimulation on the expression of SP, NK-1 and TRPV1 mRNAs during cardiac ischemia in rat. / Ding, Xiao Hui; Mountain, Deidra; Subramanian, Venkateswaran; Singh, Krishna; Williams, Carole Ann.

In: Neuroscience Letters, Vol. 424, No. 2, 07.09.2007, p. 139-144.

Research output: Contribution to journalArticle

Ding, Xiao Hui ; Mountain, Deidra ; Subramanian, Venkateswaran ; Singh, Krishna ; Williams, Carole Ann. / The effect of high cervical spinal cord stimulation on the expression of SP, NK-1 and TRPV1 mRNAs during cardiac ischemia in rat. In: Neuroscience Letters. 2007 ; Vol. 424, No. 2. pp. 139-144.
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