The effect of radiation dose on mouse skeletal muscle remodeling

Justin P. Hardee, Melissa J. Puppa, Dennis K. Fix, Song Gao, Kimbell L. Hetzler, Ted A. Bateman, James Carson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: The purpose of this study was to determine the effect of two clinically relevant radiation doses on the susceptibility of mouse skeletal muscle to remodeling. Materials and methods: Alterations in muscle morphology and regulatory signaling were examined in tibialis anterior and gastrocnemius muscles after radiation doses that differed in total biological effective dose (BED). Female C57BL/6 (8-wk) mice were randomly assigned to non-irradiated control, four fractionated doses of 4 Gy (4x4 Gy; BED 37 Gy), or a single 16 Gy dose (16 Gy; BED 100 Gy). Mice were sacrificed 2 weeks after the initial radiation exposure. Results: The 16 Gy, but not 4x4 Gy, decreased total muscle protein and RNA content. Related to muscle regeneration, both 16 Gy and 4x4 Gy increased the incidence of central nuclei containing myofibers, but only 16 Gy increased the extracellular matrix volume. However, only 4x4 Gy increased muscle 4-hydroxynonenal expression. While both 16 Gy and 4x4 Gy decreased IIB myofiber mean cross-sectional area (CSA), only 16 Gy decreased IIA myofiber CSA. 16 Gy increased the incidence of small diameter IIA and IIB myofibers, while 4x4 Gy only increased the incidence of small diameter IIB myofibers. Both treatments decreased the frequency and CSA of low succinate dehydrogenase activity (SDH) fibers. Only 16 Gy increased the incidence of small diameter myofibers having high SDH activity. Neither treatment altered muscle signaling related to protein turnover or oxidative metabolism. Conclusions: Collectively, these results demonstrate that radiation dose differentially affects muscle remodeling, and these effects appear to be related to fiber type and oxidative metabolism.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalRadiology and Oncology
Volume48
Issue number3
DOIs
StatePublished - Sep 1 2014
Externally publishedYes

Fingerprint

Radiation Effects
Skeletal Muscle
Muscles
Succinate Dehydrogenase
Incidence
Radiation
Muscle Proteins
Extracellular Matrix
Regeneration
RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

Hardee, J. P., Puppa, M. J., Fix, D. K., Gao, S., Hetzler, K. L., Bateman, T. A., & Carson, J. (2014). The effect of radiation dose on mouse skeletal muscle remodeling. Radiology and Oncology, 48(3), 247-256. https://doi.org/10.2478/raon-2014-0025

The effect of radiation dose on mouse skeletal muscle remodeling. / Hardee, Justin P.; Puppa, Melissa J.; Fix, Dennis K.; Gao, Song; Hetzler, Kimbell L.; Bateman, Ted A.; Carson, James.

In: Radiology and Oncology, Vol. 48, No. 3, 01.09.2014, p. 247-256.

Research output: Contribution to journalArticle

Hardee, JP, Puppa, MJ, Fix, DK, Gao, S, Hetzler, KL, Bateman, TA & Carson, J 2014, 'The effect of radiation dose on mouse skeletal muscle remodeling', Radiology and Oncology, vol. 48, no. 3, pp. 247-256. https://doi.org/10.2478/raon-2014-0025
Hardee JP, Puppa MJ, Fix DK, Gao S, Hetzler KL, Bateman TA et al. The effect of radiation dose on mouse skeletal muscle remodeling. Radiology and Oncology. 2014 Sep 1;48(3):247-256. https://doi.org/10.2478/raon-2014-0025
Hardee, Justin P. ; Puppa, Melissa J. ; Fix, Dennis K. ; Gao, Song ; Hetzler, Kimbell L. ; Bateman, Ted A. ; Carson, James. / The effect of radiation dose on mouse skeletal muscle remodeling. In: Radiology and Oncology. 2014 ; Vol. 48, No. 3. pp. 247-256.
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