The effect of radiopacifiers agents on pH, calcium release, radiopacity, and antimicrobial properties of different calcium hydroxide dressings

Ronald Ordinola-Zapata, Clovis Monteiro Bramante, Franklin Garcia-Godoy, Bertram Ivan Moldauer, Paloma Gagliardi Minotti, Larissa Tercília Grizzo, Marco Antonio Hungaro Duarte

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this study was to evaluate the antimicrobial activity, pH level, calcium ion release, and radiopacity of calcium hydroxide pastes associated with three radiopacifying agents (iodoform, zinc oxide, and barium sulfate). For the pH and calcium release tests, 45 acrylic teeth were utilized and immersed in ultrapure water. After 24 h, 72 h, and 7 days the solution was analyzed by using a pH meter and an atomic absorption spectrophotometer. Polyethylene tubes filled with the pastes were used to perform the radiopacity test. For the antimicrobial test, 25 dentin specimens were infected intraorally in order to induce the biofilm colonization and treated with the pastes for 7 days. The Live/Dead technique and a confocal microscope were used to obtain the ratio of live cells. Parametric and nonparametric statistical tests were performed to show differences among the groups (P<0.05). The pH analysis at 7 days showed significant differences (P<0.05) among the groups. No differences among the pastes were found in the calcium release test on the 7th day (P>0.05). The calcium hydroxide/iodoform samples had the highest radiopacity and antimicrobial activity against the biofilm-infected dentin in comparison to the other pastes (P<0.05). Calcium hydroxide mixed with 17% iodoform and 35% propylene glycol into a paste had the highest pH, calcium ion release, radiopacity, and the greatest antimicrobial action versus similar samples mixed with BaSO4 or ZnO.

Original languageEnglish (US)
Pages (from-to)620-625
Number of pages6
JournalMicroscopy Research and Technique
Volume78
Issue number7
DOIs
StatePublished - Jan 1 2015

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Calcium Hydroxide
Hydrated lime
Bandages
Ointments
hydroxides
calcium
Calcium
Biofilms
pH meters
Dentin
biofilms
Statistical tests
Spectrophotometers
Ions
Barium
Glycols
Zinc oxide
Acrylics
Propylene
Polyethylenes

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

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The effect of radiopacifiers agents on pH, calcium release, radiopacity, and antimicrobial properties of different calcium hydroxide dressings. / Ordinola-Zapata, Ronald; Bramante, Clovis Monteiro; Garcia-Godoy, Franklin; Moldauer, Bertram Ivan; Minotti, Paloma Gagliardi; Tercília Grizzo, Larissa; Duarte, Marco Antonio Hungaro.

In: Microscopy Research and Technique, Vol. 78, No. 7, 01.01.2015, p. 620-625.

Research output: Contribution to journalArticle

Ordinola-Zapata, Ronald ; Bramante, Clovis Monteiro ; Garcia-Godoy, Franklin ; Moldauer, Bertram Ivan ; Minotti, Paloma Gagliardi ; Tercília Grizzo, Larissa ; Duarte, Marco Antonio Hungaro. / The effect of radiopacifiers agents on pH, calcium release, radiopacity, and antimicrobial properties of different calcium hydroxide dressings. In: Microscopy Research and Technique. 2015 ; Vol. 78, No. 7. pp. 620-625.
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