The effects of acute and repeated cocaine injections on protein kinase C activity and isoform levels in dopaminergic brain regions

Jeffery Steketee, Lori A. Rowe, L. Judson Chandler

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The present study was designed to assess of acute and repeated cocaine exposure on protein kinase C (PKC) activity and levels of calcium-dependent isoforms of PKC in mesocorticolimbic and nigrostriatal dopamine brain regions. Animals were sacrified 2, 6 or 24 h after the challenge injection and their brains were dissected and used in PKC studies. The data demonstrated that previously reported cocaine-induced increases in PKC activity in the ventral tegmental area are transient and not associated with changes in the levels of calcium-dependent isoforms of PKC. In addition, there was a decrease in membrane-associated PKC activity, with a concomitant increase in the levels of PKCβ(I) in the medial prefrontal cortex 24 h after the last injection of cocaine. These data suggest that changes in PKC activity in the vental tegmental area may be involved in the initiation of sensitization whereas changes in PKC activity in the medial prefrontal cortex may be related to the expression of the sensitized response to cocaine.

Original languageEnglish (US)
Pages (from-to)339-347
Number of pages9
JournalNeuropharmacology
Volume37
Issue number3
DOIs
StatePublished - Mar 1 1998

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Cocaine
Protein Kinase C
Protein Isoforms
Injections
Brain
Prefrontal Cortex
Ventral Tegmental Area
Dopamine
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

The effects of acute and repeated cocaine injections on protein kinase C activity and isoform levels in dopaminergic brain regions. / Steketee, Jeffery; Rowe, Lori A.; Judson Chandler, L.

In: Neuropharmacology, Vol. 37, No. 3, 01.03.1998, p. 339-347.

Research output: Contribution to journalArticle

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