The Effects of Platelet-Rich Plasma on Halting the Progression in Porcine Intervertebral Disc Degeneration

Hongsik Cho, David C. Holt, Richard Smith, Song Ja Kim, Raymond J. Gardocki, Karen Hasty

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Disc degeneration and the subsequent herniation and/or rupture of the intervertebral disc (IVD) are due to a failure of the extracellular matrix of the annulus to contain the contents of the nucleus. This results from inadequate maintenance of the matrix components as well as the proteolytic activity of matrix metalloproteinases (MMPs) that degrade matrix molecules. Arresting progression of disc degeneration in the annulus holds greater clinical potential at this point than prevention of its onset in the nucleus. Therefore, in this study, we have therapeutic aims that would decrease levels of the cytokines and growth factors that indirectly lead to disc degeneration via stimulating MMP and increase levels of several beneficial growth factors, such as transforming growth factor-β, with the addition of platelet-rich plasma (PRP) that would stimulate cell growth and matrix synthesis. For this study, we attempted to address these imbalances of metabolism by using tumor necrosis factor-α treated annulus fibrosus cells isolated from porcine IVD tissue and incubating the cells in a growth factor rich environment with PRP. These results indicate that the PRP in vitro increased the production of the major matrix components (type II collagen and aggrecan) and decreased the inhibitory collagenase MMP-1. This application will address a therapeutic approach for intervening early in the degenerative process.

Original languageEnglish (US)
Pages (from-to)190-195
Number of pages6
JournalArtificial Organs
Volume40
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Intervertebral Disc Degeneration
Platelet-Rich Plasma
Platelets
Intercellular Signaling Peptides and Proteins
Swine
Intervertebral Disc
Matrix Metalloproteinases
Plasmas
Aggrecans
Matrix Metalloproteinase 1
Collagen Type II
Cell growth
Transforming Growth Factors
Collagenases
Metabolism
Extracellular Matrix
Rupture
Tumor Necrosis Factor-alpha
Maintenance
Collagen

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

The Effects of Platelet-Rich Plasma on Halting the Progression in Porcine Intervertebral Disc Degeneration. / Cho, Hongsik; Holt, David C.; Smith, Richard; Kim, Song Ja; Gardocki, Raymond J.; Hasty, Karen.

In: Artificial Organs, Vol. 40, No. 2, 01.02.2016, p. 190-195.

Research output: Contribution to journalArticle

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