The effects of scanning electron microscopy desiccation preparation on demineralized dentin surfaces

John D. Sterrett, Murray Marks, John Dunlap, Jerilyn Swann, Kathy Schrock

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study assessed the effect various scanning electron microscopy (SEM) desiccation preparation techniques have on a tufted collagen surface produced using an acid-burnished (rubbed) demineralization application technique. Citric acid- soaked cotton pellets (30%) were burnished on the dentin treatment region, rinsed in water, and then fixed. Four SEM desiccation preparation techniques were employed: (1) air-dried from glutaraldehyde; (2) air-dried from ethanol; (3) critical point dried from liquid carbon dioxide (control); or (4) air-dried from tetramethylsilane. Control specimens all displayed a characteristic tufted fibril surface. In all experimental groups fibrils collapsed, forming an intact, undulating nondescript surface monolayer. All air-drying SEM desiccation preparation procedures appear to cause artifactual distortion of a tufted dentin collagen surface.

Original languageEnglish (US)
Pages (from-to)403-408
Number of pages6
JournalInternational Journal of Periodontics and Restorative Dentistry
Volume35
Issue number3
DOIs
StatePublished - Jan 1 2015

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Desiccation
Dentin
Electron Scanning Microscopy
Air
Collagen
Glutaral
Carbon Dioxide
Citric Acid
Ethanol
Acids
Water

All Science Journal Classification (ASJC) codes

  • Oral Surgery
  • Periodontics

Cite this

The effects of scanning electron microscopy desiccation preparation on demineralized dentin surfaces. / Sterrett, John D.; Marks, Murray; Dunlap, John; Swann, Jerilyn; Schrock, Kathy.

In: International Journal of Periodontics and Restorative Dentistry, Vol. 35, No. 3, 01.01.2015, p. 403-408.

Research output: Contribution to journalArticle

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