The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro

Xunda A. Gibson, Archil Shartava, Jonah McIntyre, Carlos A. Monteiro, Yalin Zhang, Arvind Shah, Naomi F. Campbell, Steven Goodman

Research output: Contribution to journalArticle

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Abstract

We show that N-acetylcysteine (NAC) has the ability to cause statistically significant diminishment in the in vitro formation of irreversibly sickled cells (ISCs) at concentrations greater than 250 μmol/L. Other antioxidants, approved for human use (cysteamine, succimer, dimercaprol), were not efficacious. NAC had the ability to cause statistically significant conversion of ISCs formed in vivo back to the biconcave shape. NAC was also shown to reduce the formation of dense cells and increase the available thiols in β-actin. We showed that diminishing reduced glutathione (GSH), by treatment with 1-chloro-2,4-dinitrobenzene, resulted in increased dense cells. We conclude the NAC blocks dense cell formation and ISC formation by targeting channels involved in cellular dehydration and β-actin, respectively. The efficacy of NAC is probably due to its combined antioxidant activity and ability to increase intracellular GSH.

Original languageEnglish (US)
Pages (from-to)4373-4378
Number of pages6
JournalBlood
Volume91
Issue number11
StatePublished - Jun 1 1998
Externally publishedYes

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Reducing Agents
Acetylcysteine
Antioxidants
Cells
Actins
Succimer
Cysteamine
Dimercaprol
Dinitrochlorobenzene
Dehydration
Sulfhydryl Compounds
Glutathione
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Gibson, X. A., Shartava, A., McIntyre, J., Monteiro, C. A., Zhang, Y., Shah, A., ... Goodman, S. (1998). The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro. Blood, 91(11), 4373-4378.

The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro. / Gibson, Xunda A.; Shartava, Archil; McIntyre, Jonah; Monteiro, Carlos A.; Zhang, Yalin; Shah, Arvind; Campbell, Naomi F.; Goodman, Steven.

In: Blood, Vol. 91, No. 11, 01.06.1998, p. 4373-4378.

Research output: Contribution to journalArticle

Gibson, XA, Shartava, A, McIntyre, J, Monteiro, CA, Zhang, Y, Shah, A, Campbell, NF & Goodman, S 1998, 'The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro', Blood, vol. 91, no. 11, pp. 4373-4378.
Gibson XA, Shartava A, McIntyre J, Monteiro CA, Zhang Y, Shah A et al. The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro. Blood. 1998 Jun 1;91(11):4373-4378.
Gibson, Xunda A. ; Shartava, Archil ; McIntyre, Jonah ; Monteiro, Carlos A. ; Zhang, Yalin ; Shah, Arvind ; Campbell, Naomi F. ; Goodman, Steven. / The efficacy of reducing agents or antioxidants in blocking the formation of dense cells and irreversibly sickled cells in vitro. In: Blood. 1998 ; Vol. 91, No. 11. pp. 4373-4378.
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