The epidermal growth factor receptor is associated with phospholipase C-γ1 in meningiomas

Mahlon Johnson, Megumi Horiba, Angela R. Winnier, Carlos L. Arteaga

Research output: Contribution to journalArticle

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Abstract

In this study we evaluated phospholipase C-γ1 (PLC-γ1) expression, activity, and association with the epidermal growth factor (EGF) receptor in a series of human meningiomas as well as cultured meningioma cells. Phospholipase C-γ1 was detectable by immunoblot and immunohistochemistry in 13 of 13 meningioma specimens. Epidermal growth factor receptors were detected by immunoblot in six of nine meningiomas (67%) and by immunohistochemistry in 13 of 19 meningiomas (68%) but not in normal leptomeningeal cells. In two of three meningiomas EGF receptors and/or a 170-kd phosphotyrosine band precipitated with a PLC-γ1 antiserum. Both PLC-γ1 and EGF receptors also exhibited the same pattern of immunostaining on meningioma tissue sections. Phospholipase C-γ1 catalytic activity, measured in a PIP2 hydrolysis assay, was higher in nine EGF receptor-positive meningiomas than in six EGF receptor-negative meningiomas (P = .05; t test). Finally, treatment of cultured meningioma cells with transforming growth factor-α induced a 78% increase in PLC-γ1 catalytic activity. Thus, these data are consistent with the possibility that the EGF receptor tyrosine kinase regulates PLC-γ1 activity in native meningioma tissue.

Original languageEnglish (US)
Pages (from-to)146-153
Number of pages8
JournalHuman pathology
Volume25
Issue number2
DOIs
StatePublished - Jan 1 1994

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Type C Phospholipases
Meningioma
Epidermal Growth Factor Receptor
Cultured Cells
Immunohistochemistry
Phosphotyrosine
Transforming Growth Factors
Protein-Tyrosine Kinases
Immune Sera
Hydrolysis

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

The epidermal growth factor receptor is associated with phospholipase C-γ1 in meningiomas. / Johnson, Mahlon; Horiba, Megumi; Winnier, Angela R.; Arteaga, Carlos L.

In: Human pathology, Vol. 25, No. 2, 01.01.1994, p. 146-153.

Research output: Contribution to journalArticle

Johnson, Mahlon ; Horiba, Megumi ; Winnier, Angela R. ; Arteaga, Carlos L. / The epidermal growth factor receptor is associated with phospholipase C-γ1 in meningiomas. In: Human pathology. 1994 ; Vol. 25, No. 2. pp. 146-153.
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