The Essentiality of Decyclization of Pyrrolidonecarboxylic Acid Prior to Utilization in Protein Biosynthesis

E. Ann Rush, Carla L. McLaughlin, Alan Solomon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Pyrrolidonecarboxylic acid (PCA) is not incorporated directly into a protein polypeptide chain but can be converted into a utilizable form by a decyclase factor. The detection of radioactivity in newly synthesized protein produced by cells incubated in medium containing PCA-14C was indicative of the presence of decyclase. The biosynthetic demonstration of PCA decylase in a human plasmacytoma producing a Bence Jones protein with a cyclized glutaminyl amino-terminal residue contrasted to the absence of demonstrable decyclase in a murine plasmacytoma, which was also producing a Bence Jones protein with a cyclized glutaminyl amino terminus. These results indicate a lack of correlation between the presence of PCA decyclase and the occurrence of cyclized glutaminyl as the amino-terminal residue of a polypeptide chain.

Original languageEnglish (US)
Pages (from-to)1134-1139
Number of pages6
JournalCancer Research
Volume31
Issue number8
StatePublished - Jan 1 1971

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Pyrrolidonecarboxylic Acid
Protein Biosynthesis
Bence Jones Protein
Plasmacytoma
Peptides
Radioactivity
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

The Essentiality of Decyclization of Pyrrolidonecarboxylic Acid Prior to Utilization in Protein Biosynthesis. / Rush, E. Ann; McLaughlin, Carla L.; Solomon, Alan.

In: Cancer Research, Vol. 31, No. 8, 01.01.1971, p. 1134-1139.

Research output: Contribution to journalArticle

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