The expression of tyrosine hydroxylase and DARPP-32 in the house crow (Corvus splendens) brain

Shankhamala Sen, Pooja Parishar, Arvind Singh Pundir, Anton Reiner, Soumya Iyengar

Research output: Contribution to journalArticle

Abstract

Birds of the family Corvidae which includes diverse species such as crows, rooks, ravens, magpies, jays, and jackdaws are known for their amazing abilities at problem-solving. Since the catecholaminergic system, especially the neurotransmitter dopamine, plays a role in cognition, we decided to study the distribution of tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of catecholamines in the brain of house crows (Corvus splendens). We also studied the expression of DARPP-32 (dopamine and cAMP-regulated phosphoprotein), which is expressed in dopaminoceptive neurons. Our results demonstrated that as in other avian species, the expression of both TH and DARPP-32 was highest in the house crow striatum. The caudolateral nidopallium (NCL, the avian analogue of the mammalian prefrontal cortex) could be differentiated from the surrounding pallial regions based on a larger number of TH-positive “baskets” of fibers around neurons in this region and greater intensity of DARPP-32 staining in the neuropil in this region. House crows also possessed distinct nuclei in their brains which corresponded to song control regions in other songbirds. Whereas immunoreactivity for TH was higher in the vocal control region Area X compared to the surrounding MSt (medial striatum) in house crows, staining in RA and HVC was not as prominent. Furthermore, the arcopallial song control regions RA (nucleus robustus arcopallialis) and AId (intermediate arcopallium) were strikingly negative for DARPP-32 staining, in contrast to the surrounding arcopallium. Patterns of immunoreactivity for TH and DARPP-32 in “limbic” areas such as the hippocampus, septum, and extended amygdala have also been described.

Original languageEnglish (US)
Pages (from-to)1801-1836
Number of pages36
JournalJournal of Comparative Neurology
Volume527
Issue number11
DOIs
StatePublished - Aug 1 2019

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Crows
Tyrosine 3-Monooxygenase
Brain
Music
Staining and Labeling
Dopamine and cAMP-Regulated Phosphoprotein 32
Neurons
Songbirds
Aptitude
Neuropil
Amygdala
Prefrontal Cortex
Cognition
Catecholamines
Birds
Neurotransmitter Agents
Hippocampus
Dopamine
Enzymes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

The expression of tyrosine hydroxylase and DARPP-32 in the house crow (Corvus splendens) brain. / Sen, Shankhamala; Parishar, Pooja; Pundir, Arvind Singh; Reiner, Anton; Iyengar, Soumya.

In: Journal of Comparative Neurology, Vol. 527, No. 11, 01.08.2019, p. 1801-1836.

Research output: Contribution to journalArticle

Sen, Shankhamala ; Parishar, Pooja ; Pundir, Arvind Singh ; Reiner, Anton ; Iyengar, Soumya. / The expression of tyrosine hydroxylase and DARPP-32 in the house crow (Corvus splendens) brain. In: Journal of Comparative Neurology. 2019 ; Vol. 527, No. 11. pp. 1801-1836.
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