The G protein-coupled receptor Gpr1 and the Gα protein Gpa2 act through the cAMP-protein kinase A pathway to induce morphogenesis in Candida albicans

Mykola M. Maidan, Larissa De Rop, Joke Serneels, Simone Exler, Steffen Rupp, Helene Tournu, Johan M. Thevelein, Patrick Van Dijck

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Abstract

We investigated the role in cell morphogenesis and pathogenicity of the Candida albicans GPR1 gene, encoding the G protein-coupled receptor Gpr1. Deletion of C. albicans GPR1 has only minor effects in liquid hypha-inducing media but results in strong defects in the yeast-to-hypha transition on solid hypha-inducing media. Addition of cAMP, expression of a constitutively active allele of the Gα protein Gpa2 or of the catalytic protein kinase A subunit TPK1 restores the wild-type phenotype of the CaGPR1-deleted strain. Overexpression of HST7, encoding a component of the mitogen-activated protein kinase pathway, does not suppress the defect in filamentation. These results indicate that CaGpr1 functions upstream in the cAMP-protein kinase A (PKA) pathway. We also show that, in the presence of glucose, CaGpr1 is important for amino acid-induced transition from yeast to hyphal cells. Finally, as opposed to previous reports, we show that CaGpa2 acts downstream of CaGpr1 as activator of the cAMP-PKA pathway but that deletion of neither CaGpr1 nor CaGpa2 affects glucose-induced cAMP signaling. In contrast, the latter is abolished in strains lacking CaCdc25 or CaRas1, suggesting that the CaCdc25-CaRas1 rather than the CaGpr1-CaGpa2 module mediates glucose-induced cAMP signaling in C. albicans.

Original languageEnglish (US)
Pages (from-to)1971-1986
Number of pages16
JournalMolecular Biology of the Cell
Volume16
Issue number4
DOIs
StatePublished - Apr 1 2005

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Hyphae
G-Protein-Coupled Receptors
Cyclic AMP-Dependent Protein Kinases
Candida albicans
Morphogenesis
GTP-Binding Proteins
Glucose
Yeasts
Mitogen-Activated Protein Kinases
Virulence
Alleles
Phenotype
Amino Acids
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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The G protein-coupled receptor Gpr1 and the Gα protein Gpa2 act through the cAMP-protein kinase A pathway to induce morphogenesis in Candida albicans. / Maidan, Mykola M.; De Rop, Larissa; Serneels, Joke; Exler, Simone; Rupp, Steffen; Tournu, Helene; Thevelein, Johan M.; Van Dijck, Patrick.

In: Molecular Biology of the Cell, Vol. 16, No. 4, 01.04.2005, p. 1971-1986.

Research output: Contribution to journalArticle

Maidan, Mykola M. ; De Rop, Larissa ; Serneels, Joke ; Exler, Simone ; Rupp, Steffen ; Tournu, Helene ; Thevelein, Johan M. ; Van Dijck, Patrick. / The G protein-coupled receptor Gpr1 and the Gα protein Gpa2 act through the cAMP-protein kinase A pathway to induce morphogenesis in Candida albicans. In: Molecular Biology of the Cell. 2005 ; Vol. 16, No. 4. pp. 1971-1986.
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