The growth of Semliki Forest virus in cultured mosquito cells

ultrastructural observations: brief report

Rajendra Raghow, M. W. Davey, L. Dalgarno

Research output: Contribution to journalArticle

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Abstract

The maturation of Ross River virus (RRV) in A. albopictus cells occurs within large, membrane bound cytoplasmic inclusions as well as at the cell membrane. Late in infection, inclusions become depleted of virus and are transformed into electron lucent multivesicular bodies. It was suggested that during the establishment of the persistent infection, the contents of these inclusions are digested as a result of fusion with lysosomal microvesicles. In this report the ultrastructural morphology of A. albopictus cells infected with Semliki Forest virus (SFV) was examined. The growth kinetics of SFV in A. albopictus cells are shown. Electron microscopy of infected cells failed to show any intracellular virus specific structures at 12 hours after infection, although a substantial accumulation of extracellular virus was occasionally observed. Free nucleocapsids were not seen in infected cells at this or later times. At 24 hours, a small proportion (less than 5%) of cells contained numerous electron dense, cytoplasmic inclusions; these were not present in control cells, some inclusions contained microvesicles as well as enveloped virus. A smaller proportion of cells contained large cytoplasmic vacuoles with small, membranous spherules attached along their inner surface. These spherules bear a morphological similarity to virus particles lacking a central, electron dense nucleocapsid. The vacuoles, although present in very few cells, are similar to 'type 1 cytopathic vacuoles' previously described in SFV infected mammalian cells. No other cytopathic effects were noted in infected cells and the rate of division was the same as in control cultures.

Original languageEnglish (US)
Pages (from-to)165-168
Number of pages4
JournalARCH.GES.VIRUSFORSCH.
Volume43
Issue number1-2
StatePublished - 1973
Externally publishedYes

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Semliki forest virus
Culicidae
Cultured Cells
Growth
Vacuoles
Nucleocapsid
Inclusion Bodies
Electrons
Viruses
Ross River virus
Infection
Multivesicular Bodies
Viral Structures
Virion
Cell Division
Electron Microscopy
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

The growth of Semliki Forest virus in cultured mosquito cells : ultrastructural observations: brief report. / Raghow, Rajendra; Davey, M. W.; Dalgarno, L.

In: ARCH.GES.VIRUSFORSCH., Vol. 43, No. 1-2, 1973, p. 165-168.

Research output: Contribution to journalArticle

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