The GT-rich sequence in the U5 region of rous sarcoma virus long terminal repeat is required for transcription termination and 3' processing

P. J. Cleavinger, J. C. Kandala, Ramareddy Guntaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The sequences in the LTR of Rous sarcoma virus that are required for transcription termination and polyadenylation have been determined. A vector containing LTR-neo-LTR has been constructed and deletions in the U5 region of the downstream LTR have been made. The DNAs from wild-type and deletion mutant recombinant plasmids were introduced into QT6 cells acid G418-resistant transformants were selected. Those transformants with neo sequences in the arrangement, LTR-neo-LTR, were analyzed for transcription termination and polyadenylation by Northern blot analysis and by S1 protection experiments. The results indicate that the polyadenylation signal, AATAAA, located in the U3 region alone, is not sufficient for 3' end processing and that the sequence between +20 and +44 in the U5 region is absolutely required for transcription termination or endonucleolytic cleavage and polyadenylation.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalFolia Biologica
Volume43
Issue number4
StatePublished - Jan 1 1997

Fingerprint

Rous sarcoma virus
Polyadenylation
Terminal Repeat Sequences
Transcription
Viruses
Processing
Plasmids
Northern Blotting
Acids
DNA
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Molecular Biology
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

The GT-rich sequence in the U5 region of rous sarcoma virus long terminal repeat is required for transcription termination and 3' processing. / Cleavinger, P. J.; Kandala, J. C.; Guntaka, Ramareddy.

In: Folia Biologica, Vol. 43, No. 4, 01.01.1997, p. 153-160.

Research output: Contribution to journalArticle

@article{c207ed6f46b3490bb272e9d19c593e3d,
title = "The GT-rich sequence in the U5 region of rous sarcoma virus long terminal repeat is required for transcription termination and 3' processing",
abstract = "The sequences in the LTR of Rous sarcoma virus that are required for transcription termination and polyadenylation have been determined. A vector containing LTR-neo-LTR has been constructed and deletions in the U5 region of the downstream LTR have been made. The DNAs from wild-type and deletion mutant recombinant plasmids were introduced into QT6 cells acid G418-resistant transformants were selected. Those transformants with neo sequences in the arrangement, LTR-neo-LTR, were analyzed for transcription termination and polyadenylation by Northern blot analysis and by S1 protection experiments. The results indicate that the polyadenylation signal, AATAAA, located in the U3 region alone, is not sufficient for 3' end processing and that the sequence between +20 and +44 in the U5 region is absolutely required for transcription termination or endonucleolytic cleavage and polyadenylation.",
author = "Cleavinger, {P. J.} and Kandala, {J. C.} and Ramareddy Guntaka",
year = "1997",
month = "1",
day = "1",
language = "English (US)",
volume = "43",
pages = "153--160",
journal = "Folia Biologica",
issn = "0015-5500",
publisher = "Charles University",
number = "4",

}

TY - JOUR

T1 - The GT-rich sequence in the U5 region of rous sarcoma virus long terminal repeat is required for transcription termination and 3' processing

AU - Cleavinger, P. J.

AU - Kandala, J. C.

AU - Guntaka, Ramareddy

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The sequences in the LTR of Rous sarcoma virus that are required for transcription termination and polyadenylation have been determined. A vector containing LTR-neo-LTR has been constructed and deletions in the U5 region of the downstream LTR have been made. The DNAs from wild-type and deletion mutant recombinant plasmids were introduced into QT6 cells acid G418-resistant transformants were selected. Those transformants with neo sequences in the arrangement, LTR-neo-LTR, were analyzed for transcription termination and polyadenylation by Northern blot analysis and by S1 protection experiments. The results indicate that the polyadenylation signal, AATAAA, located in the U3 region alone, is not sufficient for 3' end processing and that the sequence between +20 and +44 in the U5 region is absolutely required for transcription termination or endonucleolytic cleavage and polyadenylation.

AB - The sequences in the LTR of Rous sarcoma virus that are required for transcription termination and polyadenylation have been determined. A vector containing LTR-neo-LTR has been constructed and deletions in the U5 region of the downstream LTR have been made. The DNAs from wild-type and deletion mutant recombinant plasmids were introduced into QT6 cells acid G418-resistant transformants were selected. Those transformants with neo sequences in the arrangement, LTR-neo-LTR, were analyzed for transcription termination and polyadenylation by Northern blot analysis and by S1 protection experiments. The results indicate that the polyadenylation signal, AATAAA, located in the U3 region alone, is not sufficient for 3' end processing and that the sequence between +20 and +44 in the U5 region is absolutely required for transcription termination or endonucleolytic cleavage and polyadenylation.

UR - http://www.scopus.com/inward/record.url?scp=0030855244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030855244&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 153

EP - 160

JO - Folia Biologica

JF - Folia Biologica

SN - 0015-5500

IS - 4

ER -