The influence of mono- and divalent cations on the cardiac metabolism of arachidonic acid

M. T. Weis, Kafait Malik

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Our previous study indicated that, in the isolated rabbit heart, perfusion with Ca2+ free Krebs Henseleit buffer (KHB) results in increased conversion of exogenous arachidonic acid to PGE2 and 6-keto-PGF, probably as the result of increased availability of substrate to cuclooxygenase. Since perfusion with Ca2+ free buffer is known to cause alterations in the cardiac content of various mono- and divalent cations, the present study was performed to determine: a) The relationship between the conversion of exogenous arachidonic acid to prostaglandins and cardiac content of Na+, K+, Ca2+ and Mg2+; and b) Whether enhanced arachidonic acid conversion to prostaglandins during Caa2+ free perfusion is due to reduced incorporation of this fatty acid into tissue lipids. Perfusion of the rabbit heart with Ca2+ free buffer produced a significant reduction in the tissue content of Na+, K+, Ca2+ and Mg2+. However, the production of 6-keto-PGF from exogenous arachidonic acid was linearly correlated with tissue Mg2+. These observations, together with our finding that perfusion with Ca2+ free KHB reduced the incorporation of [3H] arachidonic acid into tissue lipids, suggests that Ca2+ free perfusion may, by reducing the activity of arachidonyl CoA synthetase (a Mg2+ dependent enzyme), decrease the acylation of arachidonic acid into lipids, thus increasing the availability of arachidonic acid to cyclooxygenase.

Original languageEnglish (US)
Pages (from-to)707-723
Number of pages17
JournalProstaglandins
Volume37
Issue number6
DOIs
StatePublished - Jan 1 1989

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Monovalent Cations
Divalent Cations
Arachidonic Acid
Metabolism
Perfusion
Tissue
Lipids
Prostaglandins
Buffers
Rabbits
Availability
Acylation
Prostaglandin-Endoperoxide Synthases
Dinoprostone
Fatty Acids
Enzymes
Substrates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

The influence of mono- and divalent cations on the cardiac metabolism of arachidonic acid. / Weis, M. T.; Malik, Kafait.

In: Prostaglandins, Vol. 37, No. 6, 01.01.1989, p. 707-723.

Research output: Contribution to journalArticle

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