The Influence of Non Polar and Polar Molecules in Mouse Motile Cells Membranes and Pure Lipid Bilayers

Francisco Sierra Valdez, Linda S. Forero-Quintero, Patricio A. Zapata-Morin, Miguel Costas, Arturo Chavez-Reyes, Jesús C. Ruiz-Suárez

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report an experimental study of mouse sperm motility that shows chief aspects characteristic of neurons: the anesthetic (produced by tetracaine) and excitatory (produced by either caffeine or calcium) effects and their antagonic action. While tetracaine inhibits sperm motility and caffeine has an excitatory action, the combination of these two substances balance the effects, producing a motility quite similar to that of control cells. We also study the effects of these agents (anesthetic and excitatory) on the melting points of pure lipid liposomes constituted by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and dipalmitoyl phosphatidic acid (DPPA). Tetracaine induces a large fluidization of the membrane, shifting the liposomes melting transition temperature to much lower values. The effect of caffeine is null, but its addition to tetracaine-doped liposomes greatly screen the fluidization effect. A high calcium concentration stiffens pure lipid membranes and strongly reduces the effect of tetracaine. Molecular Dynamics Simulations are performed to further understand our experimental findings at the molecular level. We find a strong correlation between the effect of antagonic molecules that could explain how the mechanical properties suitable for normal cell functioning are affected and recovered.

Original languageEnglish (US)
Article numbere59364
JournalPloS one
Volume8
Issue number4
DOIs
StatePublished - Apr 2 2013

Fingerprint

Tetracaine
lipid bilayers
Lipid bilayers
Lipid Bilayers
Cell membranes
Membrane Lipids
caffeine
cell membranes
sperm motility
anesthetics
Caffeine
Molecules
Liposomes
mice
Sperm Motility
Fluidization
calcium
molecular dynamics
Freezing
melting point

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sierra Valdez, F., Forero-Quintero, L. S., Zapata-Morin, P. A., Costas, M., Chavez-Reyes, A., & Ruiz-Suárez, J. C. (2013). The Influence of Non Polar and Polar Molecules in Mouse Motile Cells Membranes and Pure Lipid Bilayers. PloS one, 8(4), [e59364]. https://doi.org/10.1371/journal.pone.0059364

The Influence of Non Polar and Polar Molecules in Mouse Motile Cells Membranes and Pure Lipid Bilayers. / Sierra Valdez, Francisco; Forero-Quintero, Linda S.; Zapata-Morin, Patricio A.; Costas, Miguel; Chavez-Reyes, Arturo; Ruiz-Suárez, Jesús C.

In: PloS one, Vol. 8, No. 4, e59364, 02.04.2013.

Research output: Contribution to journalArticle

Sierra Valdez, F, Forero-Quintero, LS, Zapata-Morin, PA, Costas, M, Chavez-Reyes, A & Ruiz-Suárez, JC 2013, 'The Influence of Non Polar and Polar Molecules in Mouse Motile Cells Membranes and Pure Lipid Bilayers', PloS one, vol. 8, no. 4, e59364. https://doi.org/10.1371/journal.pone.0059364
Sierra Valdez F, Forero-Quintero LS, Zapata-Morin PA, Costas M, Chavez-Reyes A, Ruiz-Suárez JC. The Influence of Non Polar and Polar Molecules in Mouse Motile Cells Membranes and Pure Lipid Bilayers. PloS one. 2013 Apr 2;8(4). e59364. https://doi.org/10.1371/journal.pone.0059364
Sierra Valdez, Francisco ; Forero-Quintero, Linda S. ; Zapata-Morin, Patricio A. ; Costas, Miguel ; Chavez-Reyes, Arturo ; Ruiz-Suárez, Jesús C. / The Influence of Non Polar and Polar Molecules in Mouse Motile Cells Membranes and Pure Lipid Bilayers. In: PloS one. 2013 ; Vol. 8, No. 4.
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