The inhibitory action of taxol on granulosa cell steroidogenesis is reversible

Thomas T. Chen, Pamela J. Massey, Michael Caudle

Research output: Contribution to journalArticle

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Abstract

Taxol is a novel anticancer agent extracted from the bark of Pacific yew trees. The drug has been approved by the FDA for the treatment of advanced ovarian cancer and is in clinical trials for other malignancies, including breast cancer. The goals of this study were to determine whether taxol adversely and irreversibly affects ovarian granulosa cell steroidogenesis. Cultured porcine granulosa cells were treated with taxol (0.12-12 μM) or vehicle (0.01-1% ethanol) in the absence or presence of 10-9 M hCG in a time- and dose-response study. Morphological changes were recorded every 2 h, and media were collected for the measurement of progesterone (P4) and 17 β-estradiol. Taxol suppressed both basal P4 and 17 β-estradiol production and hCG-stimulated P4 production in a time- and dose-dependent manner and drastically changed cell shape by causing disorganization of microtubule bundles and other subcellular organelles. hCG partially reversed the steroid inhibition induced by taxol. These changes are not attributed to ethanol used as the vehicle, because ethanol at higher concentrations than that present in taxol did not suppress P4 production. When taxol was removed from the culture, P4 production returned to control levels. The results of this study show that taxol causes a significant, but reversible, inhibition of granulosa cell steroidogenesis. This inhibitory effect can be partially overcome by co-treatment with hCG.

Original languageEnglish (US)
Pages (from-to)2178-2183
Number of pages6
JournalEndocrinology
Volume134
Issue number5
DOIs
StatePublished - Jan 1 1994

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Granulosa Cells
Paclitaxel
Ethanol
Estradiol
Cell Shape
Microtubules
Organelles
Antineoplastic Agents
Ovarian Neoplasms
Progesterone
Swine
Steroids
Clinical Trials
Breast Neoplasms
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

The inhibitory action of taxol on granulosa cell steroidogenesis is reversible. / Chen, Thomas T.; Massey, Pamela J.; Caudle, Michael.

In: Endocrinology, Vol. 134, No. 5, 01.01.1994, p. 2178-2183.

Research output: Contribution to journalArticle

Chen, Thomas T. ; Massey, Pamela J. ; Caudle, Michael. / The inhibitory action of taxol on granulosa cell steroidogenesis is reversible. In: Endocrinology. 1994 ; Vol. 134, No. 5. pp. 2178-2183.
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