The intermediate conductance calcium-activated potassium channel KCa3.1 regulates vascular smooth muscle cell proliferation via controlling calcium-dependent signaling

Dan Bi, Kazuyoshi Toyama, Vincent Lemaître, Jun Takai, Fan Fan, David P. Jenkins, Heike Wulff, David D. Gutterman, Frank Park, Hiroto Miura

Research output: Contribution to journalArticle

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Abstract

The intermediate conductance calcium-activated potassium channel KCa3.1 contributes to a variety of cell activation processes in pathologies such as inflammation, carcinogenesis, and vascular remodeling. We examined the electrophysiological and transcriptional mechanisms by which KCa3.1 regulates vascular smooth muscle cell (VSMC) proliferation. Platelet-derived growth factor-BB (PDGF)-induced proliferation of human coronary artery VSMCs was attenuated by lowering intracellular Ca2+ concentration ([Ca 2+]i) and was enhanced by elevating [Ca2+] i. KCa3.1 blockade or knockdown inhibited proliferation by suppressing the rise in [Ca2+]i and attenuating the expression of phosphorylated cAMP-response element-binding protein (CREB), c-Fos, and neuron-derived orphan receptor-1 (NOR-1). This antiproliferative effect was abolished by elevating [Ca2+]i. KCa3.1 overexpression induced VSMC proliferation, and potentiated PDGF-induced proliferation, by inducing CREB phosphorylation, c-Fos, and NOR-1. Pharmacological stimulation of KCa3.1 unexpectedly suppressed proliferation by abolishing the expression and activity of KCa3.1 and PDGF β-receptors and inhibiting the rise in [Ca2+]i. The stimulation also attenuated the levels of phosphorylated CREB, c-Fos, and cyclin expression. After KCa3.1 blockade, the characteristic round shape of VSMCs expressing high l-caldesmon and low calponin-1 (dedifferentiation state) was maintained, whereas KCa3.1 stimulation induced a spindle-shaped cellular appearance, with low l-caldesmon and high calponin-1. In conclusion, KCa3.1 plays an important role in VSMC proliferation via controlling Ca2+-dependent signaling pathways, and its modulation may therefore constitute a new therapeutic target for cell proliferative diseases such as atherosclerosis.

Original languageEnglish (US)
Pages (from-to)15843-15853
Number of pages11
JournalJournal of Biological Chemistry
Volume288
Issue number22
DOIs
StatePublished - May 31 2013

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Intermediate-Conductance Calcium-Activated Potassium Channels
Cyclic AMP Response Element-Binding Protein
Nuclear Receptor Subfamily 4, Group A, Member 3
Calcium Signaling
Cell proliferation
Vascular Smooth Muscle
Calmodulin-Binding Proteins
Smooth Muscle Myocytes
Muscle
Cell Proliferation
Calcium
Platelet-Derived Growth Factor Receptors
Phosphorylation
Cyclins
Pathology
Atherosclerosis
Coronary Vessels
Carcinogenesis
Chemical activation
Modulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The intermediate conductance calcium-activated potassium channel KCa3.1 regulates vascular smooth muscle cell proliferation via controlling calcium-dependent signaling. / Bi, Dan; Toyama, Kazuyoshi; Lemaître, Vincent; Takai, Jun; Fan, Fan; Jenkins, David P.; Wulff, Heike; Gutterman, David D.; Park, Frank; Miura, Hiroto.

In: Journal of Biological Chemistry, Vol. 288, No. 22, 31.05.2013, p. 15843-15853.

Research output: Contribution to journalArticle

Bi, Dan ; Toyama, Kazuyoshi ; Lemaître, Vincent ; Takai, Jun ; Fan, Fan ; Jenkins, David P. ; Wulff, Heike ; Gutterman, David D. ; Park, Frank ; Miura, Hiroto. / The intermediate conductance calcium-activated potassium channel KCa3.1 regulates vascular smooth muscle cell proliferation via controlling calcium-dependent signaling. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 22. pp. 15843-15853.
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AU - Fan, Fan

AU - Jenkins, David P.

AU - Wulff, Heike

AU - Gutterman, David D.

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AU - Miura, Hiroto

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