The involvement of vimentin in copper-induced regression of cardiomyocyte hypertrophy

Rui Li, Katherine Bourcy, Tao Wang, Miao Sun, Yujian Kang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dietary copper supplementation reverses the pressure overload-induced cardiac hypertrophy. Activation of vascular endothelial growth factor receptor-1 (VEGFR-1) and cyclic guanosine monophosphate (cGMP)-dependent protein kinase-1 (PKG-1) is required for the regression. The present study was undertaken to determine the link between VEGFR-1 and PKG-1 in copper regression of cardiomyocyte hypertrophy. Human cardiac myocytes (HCM) or primary cultures of neonatal rat cardiomyocytes were exposed to phenylephrine (PE) at a final concentration of 100 μM for 48 h to induce cell hypertrophy. Copper sulfite was added to cultures of hypertrophic cardiomyocytes at a final concentration of 5 μM elemental copper and incubated for 24 h to reverse cell hypertrophy. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis identified a 56 kDa copper-binding protein, vimentin, which was co-immunoprecipitated with VEGFR-1 and PKG-1. Copper supplementation increased vimentin levels and enhanced PKG-1 activity. Gene silencing using siRNA targeting vimentin prevented copper-induced elevation of vimentin, depressed the activity of PKG-1, and blocked the copper-induced regression of cardiomyocyte hypertrophy. This study demonstrates that vimentin is critically involved in the VEGFR-1 mediated activation of the PKG-1 signaling pathway, leading to regression of cardiomyocyte hypertrophy.

Original languageEnglish (US)
Pages (from-to)1331-1337
Number of pages7
JournalMetallomics
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Vimentin
Cardiac Myocytes
Hypertrophy
Copper
Vascular Endothelial Growth Factor Receptor-1
Chemical activation
Cyclic GMP-Dependent Protein Kinases
Sulfites
Liquid chromatography
Cardiomegaly
Gene Silencing
Phenylephrine
Dietary Supplements
Tandem Mass Spectrometry
Liquid Chromatography
Small Interfering RNA
Mass spectrometry
Rats
Genes
Pressure

All Science Journal Classification (ASJC) codes

  • Chemistry (miscellaneous)
  • Biophysics
  • Biomaterials
  • Biochemistry
  • Metals and Alloys

Cite this

The involvement of vimentin in copper-induced regression of cardiomyocyte hypertrophy. / Li, Rui; Bourcy, Katherine; Wang, Tao; Sun, Miao; Kang, Yujian.

In: Metallomics, Vol. 7, No. 9, 01.09.2015, p. 1331-1337.

Research output: Contribution to journalArticle

Li, Rui ; Bourcy, Katherine ; Wang, Tao ; Sun, Miao ; Kang, Yujian. / The involvement of vimentin in copper-induced regression of cardiomyocyte hypertrophy. In: Metallomics. 2015 ; Vol. 7, No. 9. pp. 1331-1337.
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