The level of DAL80 expression down-regulates GATA factor-mediated transcription in Saccharomyces cerevisiae

T. S. Cunningham, R. Rai, Terrance Cooper

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Nitrogen-catabolic gene expression in Saccharomyces cerevisiae is regulated by the action of four GATA family transcription factors: Gln3p and Gat1p/Nil1p are transcriptional activators, and Dal80 and Deh1p/Gzf3p are repressors. In addition to the GATA sequences situated upstream of all nitrogen catabolite repression-sensitive genes that encode enzyme and transport proteins, the promoters of the GAT1, DAL80, and DEH1 genes all contain multiple GATA sequences as well. These GATA sequences are the binding sites of the GATA family transcription factors and are hypothesized to mediate their autogenous and cross regulation. Here we show, using DAL80 fused to the carbon-regulated GAL1,10 or copper-regulated CUP1 promoter, that GAT1 expression is inversely regulated by the level of DAL80 expression, i.e., as DAL80 expression increases, GAT1 expression decreases. The amount of DAL80 expression also dictates the level at which DAL3, a gene activated almost exclusively by Gln3p, is transcribed. Gat1p was found to partially substitute for Gln3p in transcription. These data support the contention that regulation of GATA-factor gene expression is tightly and dynamically coupled. Finally, we suggest that the complicated regulatory circuit in which the GATA family transcription factors participate is probably most beneficial as cells make the transition from excess to limited nitrogen availability.

Original languageEnglish (US)
Pages (from-to)6584-6591
Number of pages8
JournalJournal of bacteriology
Volume182
Issue number23
DOIs
StatePublished - Dec 1 2000

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GATA Transcription Factors
Saccharomyces cerevisiae
Down-Regulation
Nitrogen
Genes
Catabolite Repression
Gene Expression
Copper
Carrier Proteins
Carbon
Binding Sites
Enzymes

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The level of DAL80 expression down-regulates GATA factor-mediated transcription in Saccharomyces cerevisiae. / Cunningham, T. S.; Rai, R.; Cooper, Terrance.

In: Journal of bacteriology, Vol. 182, No. 23, 01.12.2000, p. 6584-6591.

Research output: Contribution to journalArticle

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