The membrane-proximal stem region of vesicular stomatitis virus G protein confers efficient virus assembly

Clinton S. Robison, Michael Whitt

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

In this report, we show that the glycoprotein of vesicular stomatitis virus (VSV G) contains within its extracellular membrane-proximal stem (GS) a domain that is required for efficient VSV budding. To determine a minimal sequence in GS that provides for high-level virus assembly, we have generated a series of recombinant ΔG-VSVs which express chimeric glycoproteins having truncated stem sequences. The recombinant viruses having chimeras with 12 or more membrane-proximal residues of the G stem, and including the G protein transmembrane-cytoplasmic tail domains, produced near-wild-type levels of particles. In contrast, viruses encoding chimeras with shorter or no G-stem sequences produced ~10- to 20-fold less. This budding domain when present in chimeric glycoproteins also promoted their incorporation into the VSV envelope. We suggest that the G-stem budding domain promotes virus release by inducing membrane curvature at sites where virus budding occurs or by recruiting condensed nucleocapsids to sites on the plasma membrane which are competent for efficient virus budding.

Original languageEnglish (US)
Pages (from-to)2239-2246
Number of pages8
JournalJournal of Virology
Volume74
Issue number5
DOIs
StatePublished - Feb 23 2000

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Virus Release
Vesiculovirus
Virus Assembly
G-proteins
viruses
stems
Membranes
Glycoproteins
glycoproteins
chimerism
Viruses
Nucleocapsid
GTP-Binding Proteins
nucleocapsid
Cell Membrane
tail
plasma membrane
vesicular stomatitis virus G protein
virus assembly

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

The membrane-proximal stem region of vesicular stomatitis virus G protein confers efficient virus assembly. / Robison, Clinton S.; Whitt, Michael.

In: Journal of Virology, Vol. 74, No. 5, 23.02.2000, p. 2239-2246.

Research output: Contribution to journalArticle

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