The murine clan V(H) III related 7183, J606 and S107 and DNA4 families commonly encode for binding to a bacterial B cell superantigen

Stephen Cary, Meera Krishnan, Tony Marion, Gregg J. Silverman

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Superantigens, by virtue of their unconventional binding interactions with Ag receptors, can simulate a large subset of mature lymphocytes in the repertoire. Recent studies have documented that in vivo exposure to the model bacterial B cell superantigen, Staphylococcal protein A (SPA), induces large scale effects on murine B-cell clonal selection by mechanism(s) that include deletion of supra-clonal sets. While the structural bases for the immunomodulatory properties of several T-cell superantigens have been well characterized, the requirements for murine Fab-binding of SpA remain incompletely defined. To investigate these structural requirements, a series of direct binding and inhibition studies were performed with a large panel of Moabs of diverse variable region gene usage. These studies confirm previous reports that superantigen binding is completely restricted to the products of clan V(H) III-related families, that include the small S107 and J606 families, and we also demonstrated that usage of the related small DNA4 family commonly correlates with weaker binding activity. Furthermore, our results document that genes from the largest clan VH III family, 7183, commonly encode for Fab-mediated binding of SpA, while antibodies from five other VH families, J558, Q52, Sm7, VHll and VH12, did not display Fab- mediated SpA binding activity. By contributing to the essential foundation for understanding of the structural basis for binding interactions, these findings will aid interpretation of evolving observations regarding the clonal fates induced by in vivo B-cell superantigen exposure.

Original languageEnglish (US)
Pages (from-to)769-776
Number of pages8
JournalMolecular Immunology
Volume36
Issue number11-12
DOIs
StatePublished - Aug 1 1999

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Superantigens
B-Lymphocytes
Clonal Deletion
Lymphocyte Subsets
Staphylococcal Protein A
Genes
T-Lymphocytes
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

The murine clan V(H) III related 7183, J606 and S107 and DNA4 families commonly encode for binding to a bacterial B cell superantigen. / Cary, Stephen; Krishnan, Meera; Marion, Tony; Silverman, Gregg J.

In: Molecular Immunology, Vol. 36, No. 11-12, 01.08.1999, p. 769-776.

Research output: Contribution to journalArticle

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