The prevalence of platelet activating factor acetylhydrolase single nucleotide polymorphisms in relationship to necrotizing enterocolitis in Northwest Louisiana infants

Senthilkumar Sankararaman, Krishna Yanamandra, Dawn Napper, Gloria Caldito, Ramasubbareddy Dhanireddy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: Studies documented that platelet activating factor (PAF) and the enzyme platelet activating factor acetylhydrolase (PAFAH) play a very important role in the pathogenesis of neonatal necrotizing enterocolitis (NEC). In this retrospective, case-controlled pilot study, the authors investigated the prevalence of single nucleotide polymorphisms (Ile198Thr and Ala379Val) of the PAFAH gene. Subjects and methods: We screened 570 blood samples from both Caucasian and African-American preterm infants in the Northwest Louisiana population for the above mentioned PAFAH gene polymorphisms. Out of 570 infants, 36 had stage I or II NEC based on diagnostic coding, the International Classification of Diseases, 9th revision, Clinical Modification, 2009 (ICD-9-CM). The remaining infants without an ICD-9-CM diagnosis of NEC were recruited as control population. The DNA was isolated and restriction fragment length polymorphism microplate polymerase chain reaction assay was performed. Results: Variants of the PAFAH gene polymorphism (Ile198Thr and Ala379Val) frequencies were not significantly different between the infants with NEC and the control group (P value of 0.26 by either multiple logistic regression analysis or the Cochran-Mantel-Haenszel test). Conclusions: This is the first study of its kind in exploring the relationship between NEC and single nucleotide polymorphisms in the coding genes of the enzyme PAFAH. Our preliminary data demonstrated that adjusted for the effect of race, PAFAH polymorphisms (Ile198Thr and Ala379Val) have no significant effect on NEC.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalSpringerPlus
Volume2
Issue number1
DOIs
StatePublished - Aug 13 2013

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Necrotizing Enterocolitis
Platelet Activating Factor
Single Nucleotide Polymorphism
International Classification of Diseases
Genes
Enzymes
Premature Infants
Restriction Fragment Length Polymorphisms
African Americans
Population
Logistic Models
Regression Analysis
Polymerase Chain Reaction
Control Groups
DNA

All Science Journal Classification (ASJC) codes

  • General

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The prevalence of platelet activating factor acetylhydrolase single nucleotide polymorphisms in relationship to necrotizing enterocolitis in Northwest Louisiana infants. / Sankararaman, Senthilkumar; Yanamandra, Krishna; Napper, Dawn; Caldito, Gloria; Dhanireddy, Ramasubbareddy.

In: SpringerPlus, Vol. 2, No. 1, 13.08.2013, p. 1-6.

Research output: Contribution to journalArticle

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