The prognostic value of estrogen receptor immunocytochemistry (ERICA) in breast cancer does not depend on the immunostaining intensity

Mamoun Younes, Rodolfo Laucirica, Charles C. Miller, Richard W. Brown

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Estrogen receptor status (ER) is traditionally determined by the immunocytochemical assay (ERICA) utilizing a formula, the HSCORE, based on the stain intensity and the percentage of ER-positive cells (%PC). The stain intensity is difficult to control and its determination is subjective. To determine whether %PC alone can be used instead of HSCORE in determining ER status in breast cancer, frozen samples from 65 breast cancers were assayed for ER by the dextran-coated charcoal assay (DCCA), HSCORE, and %PC. %PC and HSCORE correlated well. ER status determined by any of the three methods was a significant prognostic factor. When applied to paraffin sections of a different group of 91 cancers, ER %PC and DCCA did not give significant results. We conclude that (1) %PC may be used instead of HSCORE for determining ER in tissue sections and (2) inconsistencies of the prognostic significance of ER may be due to differences in populations studied.

Original languageEnglish (US)
Pages (from-to)157-170
Number of pages14
JournalBreast Disease
Volume9
Issue number3
StatePublished - May 1 1996
Externally publishedYes

Fingerprint

Estrogen Receptors
Immunohistochemistry
Breast Neoplasms
Charcoal
Dextrans
Coloring Agents
Paraffin
Population
Neoplasms

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

The prognostic value of estrogen receptor immunocytochemistry (ERICA) in breast cancer does not depend on the immunostaining intensity. / Younes, Mamoun; Laucirica, Rodolfo; Miller, Charles C.; Brown, Richard W.

In: Breast Disease, Vol. 9, No. 3, 01.05.1996, p. 157-170.

Research output: Contribution to journalArticle

@article{836969c67cb648e2b7e9d4791f355743,
title = "The prognostic value of estrogen receptor immunocytochemistry (ERICA) in breast cancer does not depend on the immunostaining intensity",
abstract = "Estrogen receptor status (ER) is traditionally determined by the immunocytochemical assay (ERICA) utilizing a formula, the HSCORE, based on the stain intensity and the percentage of ER-positive cells ({\%}PC). The stain intensity is difficult to control and its determination is subjective. To determine whether {\%}PC alone can be used instead of HSCORE in determining ER status in breast cancer, frozen samples from 65 breast cancers were assayed for ER by the dextran-coated charcoal assay (DCCA), HSCORE, and {\%}PC. {\%}PC and HSCORE correlated well. ER status determined by any of the three methods was a significant prognostic factor. When applied to paraffin sections of a different group of 91 cancers, ER {\%}PC and DCCA did not give significant results. We conclude that (1) {\%}PC may be used instead of HSCORE for determining ER in tissue sections and (2) inconsistencies of the prognostic significance of ER may be due to differences in populations studied.",
author = "Mamoun Younes and Rodolfo Laucirica and Miller, {Charles C.} and Brown, {Richard W.}",
year = "1996",
month = "5",
day = "1",
language = "English (US)",
volume = "9",
pages = "157--170",
journal = "Breast Disease",
issn = "0888-6008",
publisher = "IOS Press",
number = "3",

}

TY - JOUR

T1 - The prognostic value of estrogen receptor immunocytochemistry (ERICA) in breast cancer does not depend on the immunostaining intensity

AU - Younes, Mamoun

AU - Laucirica, Rodolfo

AU - Miller, Charles C.

AU - Brown, Richard W.

PY - 1996/5/1

Y1 - 1996/5/1

N2 - Estrogen receptor status (ER) is traditionally determined by the immunocytochemical assay (ERICA) utilizing a formula, the HSCORE, based on the stain intensity and the percentage of ER-positive cells (%PC). The stain intensity is difficult to control and its determination is subjective. To determine whether %PC alone can be used instead of HSCORE in determining ER status in breast cancer, frozen samples from 65 breast cancers were assayed for ER by the dextran-coated charcoal assay (DCCA), HSCORE, and %PC. %PC and HSCORE correlated well. ER status determined by any of the three methods was a significant prognostic factor. When applied to paraffin sections of a different group of 91 cancers, ER %PC and DCCA did not give significant results. We conclude that (1) %PC may be used instead of HSCORE for determining ER in tissue sections and (2) inconsistencies of the prognostic significance of ER may be due to differences in populations studied.

AB - Estrogen receptor status (ER) is traditionally determined by the immunocytochemical assay (ERICA) utilizing a formula, the HSCORE, based on the stain intensity and the percentage of ER-positive cells (%PC). The stain intensity is difficult to control and its determination is subjective. To determine whether %PC alone can be used instead of HSCORE in determining ER status in breast cancer, frozen samples from 65 breast cancers were assayed for ER by the dextran-coated charcoal assay (DCCA), HSCORE, and %PC. %PC and HSCORE correlated well. ER status determined by any of the three methods was a significant prognostic factor. When applied to paraffin sections of a different group of 91 cancers, ER %PC and DCCA did not give significant results. We conclude that (1) %PC may be used instead of HSCORE for determining ER in tissue sections and (2) inconsistencies of the prognostic significance of ER may be due to differences in populations studied.

UR - http://www.scopus.com/inward/record.url?scp=0029777495&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029777495&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 157

EP - 170

JO - Breast Disease

JF - Breast Disease

SN - 0888-6008

IS - 3

ER -