The role of a recombinant fragment of laminin-332 in integrin α3β1-dependent cell binding, spreading and migration

Hironobu Yamashita, Manish Tripathi, Mark P. Harris, Shanshan Liu, Brandy Weidow, Roy Zent, Vito Quaranta

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The extracellular matrix (ECM) is thought to be an essential component of tissue scaffolding and engineering because it fulfills fundamental functions related to cell adhesion, migration, and three-dimensional organization. Natural ECM preparations, however, are challenging to work with because they are comprised of macromolecules that are large and insoluble in their functional state. Functional fragments of ECM macromolecules are a viable answer to this challenge, as demonstrated by the RGD-based engineered scaffolds, where the tri-peptide, Arg-Gly-Asp (RGD), represents the minimal functional unit of fibronectin and related ECM. Laminins (Ln) are main components of epithelial tissues, since they enter into the composition of basement membranes. Application of Ln to epithelial tissue engineering would be desirable, since they could help mimic ideal functional conditions for both lining and glandular epithelial tissues. However, functional fragments of Ln that could be used in artificial settings have not been characterized in detail. In this paper, we describe the production and application of the recombinant LG4 (rLG4) fragment of laminin-332 (Ln-332), and show that it mimics three fundamental functional properties of Ln-332: integrin-mediated cell adhesion, spreading, and migration. Adhesive structures formed by cells on rLG4 closely resemble those formed on Ln-332, as judged by microscopy-based analyses of their molecular composition. As on Ln-332, focal adhesion kinase (FAK) is phosphorylated in cells adhering to rLG4, and colocalized with other focal adhesion components. We conclude that rLG4 could be a useful substitute to recapitulate, in vitro, the tissue scaffolding properties of Ln-332.

Original languageEnglish (US)
Pages (from-to)5110-5121
Number of pages12
JournalBiomaterials
Volume31
Issue number19
DOIs
StatePublished - Jul 1 2010

Fingerprint

Integrins
Extracellular Matrix
Laminin
Cell adhesion
Tissue Scaffolds
Macromolecules
Tissue engineering
Epithelium
Tissue Engineering
Adhesion
Cell Adhesion
Cell Movement
Tissue
Scaffolds (biology)
Focal Adhesion Protein-Tyrosine Kinases
Chemical analysis
Linings
Focal Adhesions
Peptides
Adhesives

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

The role of a recombinant fragment of laminin-332 in integrin α3β1-dependent cell binding, spreading and migration. / Yamashita, Hironobu; Tripathi, Manish; Harris, Mark P.; Liu, Shanshan; Weidow, Brandy; Zent, Roy; Quaranta, Vito.

In: Biomaterials, Vol. 31, No. 19, 01.07.2010, p. 5110-5121.

Research output: Contribution to journalArticle

Yamashita, Hironobu ; Tripathi, Manish ; Harris, Mark P. ; Liu, Shanshan ; Weidow, Brandy ; Zent, Roy ; Quaranta, Vito. / The role of a recombinant fragment of laminin-332 in integrin α3β1-dependent cell binding, spreading and migration. In: Biomaterials. 2010 ; Vol. 31, No. 19. pp. 5110-5121.
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