The role of antigen specificity in the binding of murine monoclonal anti-DNA antibodies to microparticles from apoptotic cells

Anirudh J. Ullal, Tony Marion, David S. Pisetsky

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus and markers of underlying immune system disturbances. These antibodies bind to both single-stranded and double-stranded DNA, mediating pathogenesis by forming immune complexes. As shown recently, DNA in blood exists in both free and particulate forms, with DNA representing an important component of microparticles. Microparticles are membrane-bound vesicles containing nuclear molecules, released by membrane blebbing during cell death and activation. A panel of monoclonal NZB/NZW F1 anti-DNA antibodies was tested for binding to microparticles generated from apoptotic THP-1 and Jurkat cells. These studies showed that only certain anti-DNA antibodies in the panel, specific for double-stranded DNA, bound to microparticles. Binding to particles was reduced by soluble DNA or DNase treatment. Together, these results indicate that particle binding is a feature of only certain anti-DNA antibodies, reflecting immunochemical properties of the antibodies and the nature of the exposed DNA antigens.

Original languageEnglish (US)
Pages (from-to)178-187
Number of pages10
JournalClinical Immunology
Volume154
Issue number2
DOIs
StatePublished - Jan 1 2014

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Monoclonal Antibodies
Antigens
Antibodies
DNA
Jurkat Cells
Membranes
Deoxyribonucleases
Blister
Antigen-Antibody Complex
Systemic Lupus Erythematosus
Immune System
Cell Death

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

The role of antigen specificity in the binding of murine monoclonal anti-DNA antibodies to microparticles from apoptotic cells. / Ullal, Anirudh J.; Marion, Tony; Pisetsky, David S.

In: Clinical Immunology, Vol. 154, No. 2, 01.01.2014, p. 178-187.

Research output: Contribution to journalArticle

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