The role of platelet-derived growth factor in intraluminal stented graft healing

M. P. Ombrellaro, Scott Stevens, D. O. Schaeffer, Michael Freeman, J. Sciarrotta, R. Carroll, Mitchell Goldman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: Intraluminally placed polytetrafluoroethylene grafts are associated with enhanced graft endothelialization and diminished intimal hyperplasia when compared with interposition grafts. This study determined the role of platelet-derived growth factor in intraluminal graft healing. STUDY DESIGN: Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (control, n=15) or intraluminal stented (n=15) grafting. Grafts were explanted at 1, 3, and 6 weeks. The percent of graft area endothelialization and intima to media height ratios were calculated. By using protein electrophoresis and the Western blot technique, platelet-derived growth factor, identified by immunolabeling with anti- platelet-derived growth factor antibody, was isolated from proximal, mid-, and distal graft regions and was quantified using densitometry. RESULTS: Graft area endothelialization was 0±3.3 percent, 2.3±3.3 percent, and 19.0±3.3 percent for 1-, 3-, and 6-week controls; and 4.7±3.7 percent, 30.5±3.3 percent, and 86.8±3.3 percent for 1-, 3-, and 6-week stented grafts. Endothelialization was greater in stented grafts at 3 and 6 weeks (p<.01). Proximal anastomosis intima to media height ratios were 1.61±0.15, 1.54±0.14, and 1.48±0.15 for 1-, 3-, and 6-week control grafts, and 0.42±0.18, 0.41±0.15, and 0.47±0.14 for 1-, 3-, and 6-week stented grafts. Similar intima to media height ratio values were present at the distal anastomosis. Lower intima to media height ratios were observed in all stented grafts (p<.01). The platelet-derived growth factor content at 1-, 3-, and 6- weeks was lower in all stented grafts when compared with controls. The content of platelet-derived growth factor was greatest in 3-week controls, with a significant difference noted in the mid-graft region (p<.05). The content of platelet-derived growth factor remained stable in all stented graft regions over 6 weeks. An inverse correlation between stented graft platelet-derived growth factor content and endothelialization (r=-0.43) and a positive correlation with proximal anastomotic intimal hyperplasia (r=0.73) were identified. CONCLUSIONS: Lower platelet-derived growth factor content is associated with decreased intimal hyperplasia and improved healing in intra- arterial polytetrafluoroethylene grafts.

Original languageEnglish (US)
Pages (from-to)49-57
Number of pages9
JournalJournal of the American College of Surgeons
Volume184
Issue number1
StatePublished - Jan 11 1997

Fingerprint

Platelet-Derived Growth Factor
Transplants
Tunica Intima
Polytetrafluoroethylene
Hyperplasia
Densitometry
Abdominal Aorta

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

The role of platelet-derived growth factor in intraluminal stented graft healing. / Ombrellaro, M. P.; Stevens, Scott; Schaeffer, D. O.; Freeman, Michael; Sciarrotta, J.; Carroll, R.; Goldman, Mitchell.

In: Journal of the American College of Surgeons, Vol. 184, No. 1, 11.01.1997, p. 49-57.

Research output: Contribution to journalArticle

@article{d323065d8f784f14891e193dc18f0399,
title = "The role of platelet-derived growth factor in intraluminal stented graft healing",
abstract = "BACKGROUND: Intraluminally placed polytetrafluoroethylene grafts are associated with enhanced graft endothelialization and diminished intimal hyperplasia when compared with interposition grafts. This study determined the role of platelet-derived growth factor in intraluminal graft healing. STUDY DESIGN: Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (control, n=15) or intraluminal stented (n=15) grafting. Grafts were explanted at 1, 3, and 6 weeks. The percent of graft area endothelialization and intima to media height ratios were calculated. By using protein electrophoresis and the Western blot technique, platelet-derived growth factor, identified by immunolabeling with anti- platelet-derived growth factor antibody, was isolated from proximal, mid-, and distal graft regions and was quantified using densitometry. RESULTS: Graft area endothelialization was 0±3.3 percent, 2.3±3.3 percent, and 19.0±3.3 percent for 1-, 3-, and 6-week controls; and 4.7±3.7 percent, 30.5±3.3 percent, and 86.8±3.3 percent for 1-, 3-, and 6-week stented grafts. Endothelialization was greater in stented grafts at 3 and 6 weeks (p<.01). Proximal anastomosis intima to media height ratios were 1.61±0.15, 1.54±0.14, and 1.48±0.15 for 1-, 3-, and 6-week control grafts, and 0.42±0.18, 0.41±0.15, and 0.47±0.14 for 1-, 3-, and 6-week stented grafts. Similar intima to media height ratio values were present at the distal anastomosis. Lower intima to media height ratios were observed in all stented grafts (p<.01). The platelet-derived growth factor content at 1-, 3-, and 6- weeks was lower in all stented grafts when compared with controls. The content of platelet-derived growth factor was greatest in 3-week controls, with a significant difference noted in the mid-graft region (p<.05). The content of platelet-derived growth factor remained stable in all stented graft regions over 6 weeks. An inverse correlation between stented graft platelet-derived growth factor content and endothelialization (r=-0.43) and a positive correlation with proximal anastomotic intimal hyperplasia (r=0.73) were identified. CONCLUSIONS: Lower platelet-derived growth factor content is associated with decreased intimal hyperplasia and improved healing in intra- arterial polytetrafluoroethylene grafts.",
author = "Ombrellaro, {M. P.} and Scott Stevens and Schaeffer, {D. O.} and Michael Freeman and J. Sciarrotta and R. Carroll and Mitchell Goldman",
year = "1997",
month = "1",
day = "11",
language = "English (US)",
volume = "184",
pages = "49--57",
journal = "Journal of the American College of Surgeons",
issn = "1072-7515",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - The role of platelet-derived growth factor in intraluminal stented graft healing

AU - Ombrellaro, M. P.

AU - Stevens, Scott

AU - Schaeffer, D. O.

AU - Freeman, Michael

AU - Sciarrotta, J.

AU - Carroll, R.

AU - Goldman, Mitchell

PY - 1997/1/11

Y1 - 1997/1/11

N2 - BACKGROUND: Intraluminally placed polytetrafluoroethylene grafts are associated with enhanced graft endothelialization and diminished intimal hyperplasia when compared with interposition grafts. This study determined the role of platelet-derived growth factor in intraluminal graft healing. STUDY DESIGN: Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (control, n=15) or intraluminal stented (n=15) grafting. Grafts were explanted at 1, 3, and 6 weeks. The percent of graft area endothelialization and intima to media height ratios were calculated. By using protein electrophoresis and the Western blot technique, platelet-derived growth factor, identified by immunolabeling with anti- platelet-derived growth factor antibody, was isolated from proximal, mid-, and distal graft regions and was quantified using densitometry. RESULTS: Graft area endothelialization was 0±3.3 percent, 2.3±3.3 percent, and 19.0±3.3 percent for 1-, 3-, and 6-week controls; and 4.7±3.7 percent, 30.5±3.3 percent, and 86.8±3.3 percent for 1-, 3-, and 6-week stented grafts. Endothelialization was greater in stented grafts at 3 and 6 weeks (p<.01). Proximal anastomosis intima to media height ratios were 1.61±0.15, 1.54±0.14, and 1.48±0.15 for 1-, 3-, and 6-week control grafts, and 0.42±0.18, 0.41±0.15, and 0.47±0.14 for 1-, 3-, and 6-week stented grafts. Similar intima to media height ratio values were present at the distal anastomosis. Lower intima to media height ratios were observed in all stented grafts (p<.01). The platelet-derived growth factor content at 1-, 3-, and 6- weeks was lower in all stented grafts when compared with controls. The content of platelet-derived growth factor was greatest in 3-week controls, with a significant difference noted in the mid-graft region (p<.05). The content of platelet-derived growth factor remained stable in all stented graft regions over 6 weeks. An inverse correlation between stented graft platelet-derived growth factor content and endothelialization (r=-0.43) and a positive correlation with proximal anastomotic intimal hyperplasia (r=0.73) were identified. CONCLUSIONS: Lower platelet-derived growth factor content is associated with decreased intimal hyperplasia and improved healing in intra- arterial polytetrafluoroethylene grafts.

AB - BACKGROUND: Intraluminally placed polytetrafluoroethylene grafts are associated with enhanced graft endothelialization and diminished intimal hyperplasia when compared with interposition grafts. This study determined the role of platelet-derived growth factor in intraluminal graft healing. STUDY DESIGN: Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (control, n=15) or intraluminal stented (n=15) grafting. Grafts were explanted at 1, 3, and 6 weeks. The percent of graft area endothelialization and intima to media height ratios were calculated. By using protein electrophoresis and the Western blot technique, platelet-derived growth factor, identified by immunolabeling with anti- platelet-derived growth factor antibody, was isolated from proximal, mid-, and distal graft regions and was quantified using densitometry. RESULTS: Graft area endothelialization was 0±3.3 percent, 2.3±3.3 percent, and 19.0±3.3 percent for 1-, 3-, and 6-week controls; and 4.7±3.7 percent, 30.5±3.3 percent, and 86.8±3.3 percent for 1-, 3-, and 6-week stented grafts. Endothelialization was greater in stented grafts at 3 and 6 weeks (p<.01). Proximal anastomosis intima to media height ratios were 1.61±0.15, 1.54±0.14, and 1.48±0.15 for 1-, 3-, and 6-week control grafts, and 0.42±0.18, 0.41±0.15, and 0.47±0.14 for 1-, 3-, and 6-week stented grafts. Similar intima to media height ratio values were present at the distal anastomosis. Lower intima to media height ratios were observed in all stented grafts (p<.01). The platelet-derived growth factor content at 1-, 3-, and 6- weeks was lower in all stented grafts when compared with controls. The content of platelet-derived growth factor was greatest in 3-week controls, with a significant difference noted in the mid-graft region (p<.05). The content of platelet-derived growth factor remained stable in all stented graft regions over 6 weeks. An inverse correlation between stented graft platelet-derived growth factor content and endothelialization (r=-0.43) and a positive correlation with proximal anastomotic intimal hyperplasia (r=0.73) were identified. CONCLUSIONS: Lower platelet-derived growth factor content is associated with decreased intimal hyperplasia and improved healing in intra- arterial polytetrafluoroethylene grafts.

UR - http://www.scopus.com/inward/record.url?scp=0031014599&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031014599&partnerID=8YFLogxK

M3 - Article

VL - 184

SP - 49

EP - 57

JO - Journal of the American College of Surgeons

JF - Journal of the American College of Surgeons

SN - 1072-7515

IS - 1

ER -