The role of Syk in peripheral T cells

Jeoung Eun Park, Sirshendu Majumdar, David Brand, Edward F. Rosloniec, Ae-Kyung Yi, John M. Stuart, Andrew Kang, Linda Myers

Research output: Contribution to journalArticle

Abstract

The aim of this study was to understand how Syk affects peripheral T cell function. T cells from Syk−/− chimeric mice and DR1 Sykfl/fl CD4cre conditional mice gave strong CD3-induced Th1, Th2, and Th17 cytokine responses. However, an altered peptide ligand (APL) of human CII (256–276) with two substitutions (F263N, E266D), also called A12, elicited only Th2 cytokine responses from Sykfl/fl T cells but not Sykfl/fl-CD4cre T cells. Western blots revealed a marked increase in the phosphorylation of Syk, JNK and p38 upon A12/DR1 activation in WT or Sykfl/fl T cells but not in Sykfl/flCD4-cre cells. We demonstrate that Syk is required for the APL- induction of suppressive cytokines. Chemical Syk inhibitors blocked activation of GATA-3 by peptide A12/DR1. In conclusion, this study provides novel insights into the role that Syk plays in directing T cell activity, and may shape therapeutic approaches for autoimmune diseases.

Original languageEnglish (US)
Pages (from-to)50-57
Number of pages8
JournalClinical Immunology
Volume192
DOIs
StatePublished - Jul 1 2018

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T-Lymphocytes
compound A 12
Cytokines
Peptides
Ligands
Autoimmune Diseases
Western Blotting
Phosphorylation
Therapeutics

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

The role of Syk in peripheral T cells. / Park, Jeoung Eun; Majumdar, Sirshendu; Brand, David; Rosloniec, Edward F.; Yi, Ae-Kyung; Stuart, John M.; Kang, Andrew; Myers, Linda.

In: Clinical Immunology, Vol. 192, 01.07.2018, p. 50-57.

Research output: Contribution to journalArticle

Park JE, Majumdar S, Brand D, Rosloniec EF, Yi A-K, Stuart JM et al. The role of Syk in peripheral T cells. Clinical Immunology. 2018 Jul 1;192:50-57. https://doi.org/10.1016/j.clim.2018.04.007
Park, Jeoung Eun ; Majumdar, Sirshendu ; Brand, David ; Rosloniec, Edward F. ; Yi, Ae-Kyung ; Stuart, John M. ; Kang, Andrew ; Myers, Linda. / The role of Syk in peripheral T cells. In: Clinical Immunology. 2018 ; Vol. 192. pp. 50-57.
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