The roof plate regulates cerebellar cell-type specification and proliferation

Viktor Chizhikov, Anne G. Lindgren, D. Spencer Currle, Matthew F. Rose, Edwin S. Monuki, Kathleen J. Millen

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

During embryogenesis, the isthmic organizer, a well-described signaling center at the junction of the mid-hindbrain, establishes the cerebellar territory along the anterior/posterior axis of the neural tube. Mechanisms specifying distinct populations within the early cerebellar anlage are less defined. Using a newly developed gene expression map of the early cerebellar anlage, we demonstrate that secreted signals from the rhombomere 1 roof plate are both necessary and sufficient for specification of the adjacent cerebellar rhombic lip and its derivative fates. Surprisingly, we show that the roof plate is not absolutely required for initial specification of more distal cerebellar cell fates, but rather regulates progenitor proliferation and cell position within the cerebellar anlage. Thus, in addition to the isthmus, the roof plate represents an important signaling center controlling multiple aspects of cerebellar patterning.

Original languageEnglish (US)
Pages (from-to)2793-2804
Number of pages12
JournalDevelopment
Volume133
Issue number15
DOIs
StatePublished - Aug 1 2006

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Rhombencephalon
Neural Tube
Lip
Embryonic Development
Stem Cells
Cell Proliferation
Gene Expression
Population

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Chizhikov, V., Lindgren, A. G., Currle, D. S., Rose, M. F., Monuki, E. S., & Millen, K. J. (2006). The roof plate regulates cerebellar cell-type specification and proliferation. Development, 133(15), 2793-2804. https://doi.org/10.1242/dev.02441

The roof plate regulates cerebellar cell-type specification and proliferation. / Chizhikov, Viktor; Lindgren, Anne G.; Currle, D. Spencer; Rose, Matthew F.; Monuki, Edwin S.; Millen, Kathleen J.

In: Development, Vol. 133, No. 15, 01.08.2006, p. 2793-2804.

Research output: Contribution to journalArticle

Chizhikov, V, Lindgren, AG, Currle, DS, Rose, MF, Monuki, ES & Millen, KJ 2006, 'The roof plate regulates cerebellar cell-type specification and proliferation', Development, vol. 133, no. 15, pp. 2793-2804. https://doi.org/10.1242/dev.02441
Chizhikov V, Lindgren AG, Currle DS, Rose MF, Monuki ES, Millen KJ. The roof plate regulates cerebellar cell-type specification and proliferation. Development. 2006 Aug 1;133(15):2793-2804. https://doi.org/10.1242/dev.02441
Chizhikov, Viktor ; Lindgren, Anne G. ; Currle, D. Spencer ; Rose, Matthew F. ; Monuki, Edwin S. ; Millen, Kathleen J. / The roof plate regulates cerebellar cell-type specification and proliferation. In: Development. 2006 ; Vol. 133, No. 15. pp. 2793-2804.
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