The steroidogenic and morphological effects of paclitaxel on cultured ovarian cancer cells

Mark R. Cameron, Michael Caudle, William R. Sullivan, John J. Peluso, Jay Wimalasena

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have studied the biochemical effects of paclitaxel (trade name Taxol®) in three ovarian cancer (OV Ca) cell lines and JEG-3 choriocarcinoma cells. Paclitaxel (1 μg/ml) was cytotoxic to approximately 80% of OV Ca cells, and the ED50 ranged from 6 to 9 ng/ml. Paclitaxel was also cytotoxic to JEG-3 cells (ED50 40 ng/ml), but even at 1 μg/ml, 40-50% of the cells survived paclitaxel treatment. Paclitaxel increased 17 β-estradiol secretion 3-4 fold in all three OV Ca cell lines with an ED50 range of 3-13 ng/ml. Similarly, paclitaxel increased estradiol secretion from JEG cells with an ED50 of 50 ng/ml. Colchicine also increased estradiol secretion significantly from OV Ca cells at 2 μM while reducing cell number approximately 40% (β-lumicolchicine, an inactive isomer, was ineffective at this concentration). Paclitaxel (1 μg/ml) treatment of BR OV Ca cells produced alterations in morphology leading to "rounding" of cells within 6 h of treatment. Simultaneously, paclitaxel increased immunohistochemical staining for aromatase, and this increase was coincident with morphological alterations. The present results demonstrate that low concentrations of paclitaxel can have significant steroidogenic, as well as cytotoxic, effects on OV Ca cells, suggesting that paclitaxel may activate signal transduction pathways in addition to disrupting microtubule function. These findings further suggest that paclitaxel could be efficacious at submicromolar concentrations.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalOncology Research
Volume7
Issue number3-4
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

Paclitaxel
Ovarian Neoplasms
Estradiol
Cell Line
Choriocarcinoma
Aromatase
Colchicine
Microtubules
Names
Signal Transduction
Cell Count
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Cameron, M. R., Caudle, M., Sullivan, W. R., Peluso, J. J., & Wimalasena, J. (1995). The steroidogenic and morphological effects of paclitaxel on cultured ovarian cancer cells. Oncology Research, 7(3-4), 145-156.

The steroidogenic and morphological effects of paclitaxel on cultured ovarian cancer cells. / Cameron, Mark R.; Caudle, Michael; Sullivan, William R.; Peluso, John J.; Wimalasena, Jay.

In: Oncology Research, Vol. 7, No. 3-4, 01.12.1995, p. 145-156.

Research output: Contribution to journalArticle

Cameron, MR, Caudle, M, Sullivan, WR, Peluso, JJ & Wimalasena, J 1995, 'The steroidogenic and morphological effects of paclitaxel on cultured ovarian cancer cells', Oncology Research, vol. 7, no. 3-4, pp. 145-156.
Cameron MR, Caudle M, Sullivan WR, Peluso JJ, Wimalasena J. The steroidogenic and morphological effects of paclitaxel on cultured ovarian cancer cells. Oncology Research. 1995 Dec 1;7(3-4):145-156.
Cameron, Mark R. ; Caudle, Michael ; Sullivan, William R. ; Peluso, John J. ; Wimalasena, Jay. / The steroidogenic and morphological effects of paclitaxel on cultured ovarian cancer cells. In: Oncology Research. 1995 ; Vol. 7, No. 3-4. pp. 145-156.
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