The taurine transporter

Mechanisms of regulation

Xiaobin Han, A. B. Patters, D. P. Jones, I. Zelikovic, R. W. Chesney

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Taurine transport undergoes an adaptive response to changes in taurine availability. Unlike most amino acids, taurine is not metabolized or incorporated into protein but remains free in the intracellular water. Most amino acids are reabsorbed at rates of 98-99%, but reabsorption of taurine may range from 40% to 99.5%. Factors that influence taurine accumulation include ionic environment, electrochemical charge, and post-translational and transcriptional factors. Among these are protein kinase C (PKC) activation and transactivation or repression by proto-oncogenes such as WT1, c-Jun, c-Myb and p53. Renal adaptive regulation of the taurine transporter (TauT) was studied in vivo and in vitro. Site-directed mutagenesis and the oocyte expression system were used to study post-translational regulation of the TauT by PKC. Reporter genes and Northern and Western blots were used to study transcriptional regulation of the taurine transporter gene (TauT). We demonstrated that (i) the body pool of taurine is controlled through renal adaptive regulation of TauT in response to taurine availability; (ii) ionic environment, electrochemical charge, pH, and developmental ontogeny influence renal taurine accumulation; (iii) the fourth segment of TauT is involved in the gating of taurine across the cell membrane, which is controlled by PKC phosphorylation of serine 322 at the post-translational level; (iv) expression of TauT is repressed by the p53 tumour suppressor gene and is transactivated by proto-oncogenes such as WT1, c-Jun, and c-Myb; and (v) over-expression of TauT protects renal cells from cisplatin-induced nephrotoxicity.

Original languageEnglish (US)
Pages (from-to)61-73
Number of pages13
JournalActa Physiologica
Volume187
Issue number1-2
DOIs
StatePublished - May 1 2006

Fingerprint

Taurine
Protein Kinase C
Kidney
Proto-Oncogenes
taurine transporter
Amino Acids
Site-Directed Mutagenesis
Tumor Suppressor Genes
Reporter Genes
Northern Blotting
Serine
Cisplatin
Transcriptional Activation
Oocytes
Western Blotting
Phosphorylation
Cell Membrane
Water

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Han, X., Patters, A. B., Jones, D. P., Zelikovic, I., & Chesney, R. W. (2006). The taurine transporter: Mechanisms of regulation. Acta Physiologica, 187(1-2), 61-73. https://doi.org/10.1111/j.1748-1716.2006.01573.x

The taurine transporter : Mechanisms of regulation. / Han, Xiaobin; Patters, A. B.; Jones, D. P.; Zelikovic, I.; Chesney, R. W.

In: Acta Physiologica, Vol. 187, No. 1-2, 01.05.2006, p. 61-73.

Research output: Contribution to journalArticle

Han, X, Patters, AB, Jones, DP, Zelikovic, I & Chesney, RW 2006, 'The taurine transporter: Mechanisms of regulation', Acta Physiologica, vol. 187, no. 1-2, pp. 61-73. https://doi.org/10.1111/j.1748-1716.2006.01573.x
Han X, Patters AB, Jones DP, Zelikovic I, Chesney RW. The taurine transporter: Mechanisms of regulation. Acta Physiologica. 2006 May 1;187(1-2):61-73. https://doi.org/10.1111/j.1748-1716.2006.01573.x
Han, Xiaobin ; Patters, A. B. ; Jones, D. P. ; Zelikovic, I. ; Chesney, R. W. / The taurine transporter : Mechanisms of regulation. In: Acta Physiologica. 2006 ; Vol. 187, No. 1-2. pp. 61-73.
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