The tissue-specific, compensatory expression of cyclooxygenase-1 and -2 in transgenic mice

Jianyi Zhang, Sarita Goorha, Rajendra Raghow, Leslie R. Ballou

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Prostaglandins are essential regulators of tissue homeostasis, reproduction and inflammation. We have recently shown that cells derived from cyclooxygenase (COX)-deficient mice express higher, compensatory levels of the remaining COX isozyme [Kirtikara et al., J. Exp. Med., 187, 517 (1998)]. To assess this compensatory expression phenomenon in vivo, we quantified COX-1 and COX-2 mRNA levels in various organs of COX-1- and COX-2-ablated mice using a reverse transcriptase-polymerase chain reaction (RT-PCR) method. We found that COX-1 and COX-2 mRNAs in the brains of COX-ablated mice were elevated > 2-fold compared with wild-type (WT) animals. COX-2 mRNA was enhanced ≈2-fold in the kidneys and stomachs of COX-1-deficient mice while COX-1 expression remained unchanged. Conversely, the livers of COX-2-deficient mice expressed 15-fold higher COX-1 mRNA levels, while hepatic COX-2 mRNA levels were not significantly altered in the COX-1-ablated mice. Steady state levels of COX-1 and COX-2 mRNAs in the hearts, lungs and spleens of WT, COX-1- and COX-2-deficient mice were indistinguishable from each other. Peritoneal macrophages isolated from COX-1- and COX-2-ablated mice also expressed significantly higher steady-state levels of cytoplasmic phospholipase A2 and 5-lipooxygenase mRNAs suggesting a global upregulation of eicosanoid biosynthetic pathways in COX-deficient mice. These data suggest that expression of both COX-1 and COX-2 can be re-programmed to compensate for the lack of both alleles of the alternate COX gene in transgenic mice.

Original languageEnglish (US)
Pages (from-to)121-135
Number of pages15
JournalProstaglandins and Other Lipid Mediators
Volume67
Issue number2
DOIs
StatePublished - Apr 3 2002

Fingerprint

Cyclooxygenase 1
Cyclooxygenase 2
Transgenic Mice
Tissue
Prostaglandin-Endoperoxide Synthases
Messenger RNA
Tissue homeostasis
Wild Animals
Eicosanoids
Macrophages
Liver
Phospholipases A2
Polymerase chain reaction
RNA-Directed DNA Polymerase
Biosynthetic Pathways
Peritoneal Macrophages
Reverse Transcriptase Polymerase Chain Reaction
Isoenzymes
Prostaglandins
Reproduction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

Cite this

The tissue-specific, compensatory expression of cyclooxygenase-1 and -2 in transgenic mice. / Zhang, Jianyi; Goorha, Sarita; Raghow, Rajendra; Ballou, Leslie R.

In: Prostaglandins and Other Lipid Mediators, Vol. 67, No. 2, 03.04.2002, p. 121-135.

Research output: Contribution to journalArticle

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