The UGA4 UAS(NTR) site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae

T. S. Cunningham, R. A. Dorrington, Terrance Cooper

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Abstract

Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the γ-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA- containing sequences (URS(GATA)) in vitro, and a pair of GATA-containing sequences (UAS(NTR)) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.

Original languageEnglish (US)
Pages (from-to)4718-4725
Number of pages8
JournalJournal of Bacteriology
Volume176
Issue number15
DOIs
StatePublished - Jan 1 1994

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Protein Binding
Transcriptional Activation
Saccharomyces cerevisiae
Proteins
Allantoin
Aminobutyrates
Zinc Fingers
Nitrogen
Genes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

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title = "The UGA4 UAS(NTR) site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae",
abstract = "Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the γ-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA- containing sequences (URS(GATA)) in vitro, and a pair of GATA-containing sequences (UAS(NTR)) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.",
author = "Cunningham, {T. S.} and Dorrington, {R. A.} and Terrance Cooper",
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T1 - The UGA4 UAS(NTR) site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding in Saccharomyces cerevisiae

AU - Cunningham, T. S.

AU - Dorrington, R. A.

AU - Cooper, Terrance

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the γ-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA- containing sequences (URS(GATA)) in vitro, and a pair of GATA-containing sequences (UAS(NTR)) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.

AB - Expression of the nitrogen catabolic genes in Saccharomyces cerevisiae, including those of the γ-aminobutyric acid (UGA) and allantoin (DAL) pathways, is regulated positively by the GLN3 protein and negatively by the DAL80 protein. The deduced sequences of the DAL80 and GLN3 proteins contain a zinc finger motif homologous to those shown to bind GATA sequences. In addition, DAL80 protein has been directly shown to bind to a pair of GATA- containing sequences (URS(GATA)) in vitro, and a pair of GATA-containing sequences (UAS(NTR)) is required for GLN3-dependent transcriptional activation in a heterologous expression vector. We demonstrate here that the GATA-containing sites upstream of UGA4 required for optimal GLN3-dependent transcriptional activation also mediate DAL80 protein binding in vitro and DAL80-responsive regulation in vivo.

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