The uptake of cyclic AMP by human erythrocytes and its effect on membrane phosphorylation

Edwin Thomas, Lloyd E. King, Martin Morrison

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The effects of time and cyclic AMP concentration on cyclic AMP uptake and membrane phosphorylation were studied using intact human erythrocytes. The rate of uptake of cyclic [3H]AMP was nearly linear with respect to cyclic AMP concentration. The amount taken up was small compared to the extracellular cyclic AMP concentration, but was sufficient to significantly increase the intracellular cyclic AMP concentration. Incubation with cyclic AMP resulted in increased incorporation of 32Pi into several phosphorylated membrane peptides of the intact erythrocytes. Although cyclic AMP altered the distribution of radioactivity among the membrane components, the total amount of incorporation was not increased. The effect of cyclic AMP on phosphorylation of membrane peptides was observed with extracellular cyclic AMP concentrations as low as 1 μm and was most pronounced in incubations of 1 to 4 h. These results indicate that cyclic AMP can enter erythrocytes in sufficient amounts to alter the activity of cyclic AMP-dependent protein kinases, or to alter the rate of turnover of certain phosphorylated membrane peptides.

Original languageEnglish (US)
Pages (from-to)459-464
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume196
Issue number2
DOIs
StatePublished - Jan 1 1979

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Phosphorylation
Cyclic AMP
Erythrocytes
Membranes
Peptides
Radioactivity
Cyclic AMP-Dependent Protein Kinases

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

The uptake of cyclic AMP by human erythrocytes and its effect on membrane phosphorylation. / Thomas, Edwin; King, Lloyd E.; Morrison, Martin.

In: Archives of Biochemistry and Biophysics, Vol. 196, No. 2, 01.01.1979, p. 459-464.

Research output: Contribution to journalArticle

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