The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UAS(NTR) element in Saccharomyces cerevisiae

J. A. Coffman, H. M. El Berry, Terrance Cooper

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis- acting UAS(NTR) element and trans-acting GLN3. Here, we extend the analysis to include the response of their expression to deletion of the URE2 locus. The expression of these nitrogen catabolic genes becomes, to various degrees, NCR insensitive in the ure2 deletion. This response is shown to be mediated through the GATAA-containing UAS(NTR) element and supports the current idea that the NCR regulatory circuit involves the following steps: environmental signal→URE2→GLN3→UAS(NTR) operation→NCR-sensitive gene expression. The various responses of the nitrogen catabolic genes' expression to deletion of the URE2 locus also indicate that not all NCR is mediated through URE2.

Original languageEnglish (US)
Pages (from-to)7476-7483
Number of pages8
JournalJournal of bacteriology
Volume176
Issue number24
DOIs
StatePublished - Jan 1 1994

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Saccharomyces cerevisiae
Nitrogen
Catabolite Repression
Gene Expression
Proteins
Asparagine
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UAS(NTR) element in Saccharomyces cerevisiae. / Coffman, J. A.; El Berry, H. M.; Cooper, Terrance.

In: Journal of bacteriology, Vol. 176, No. 24, 01.01.1994, p. 7476-7483.

Research output: Contribution to journalArticle

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