The urea amidolyase (DUR1,2) gene of saccharomyces cerevisiae

Francis S. Genbauffe, Terrance Cooper

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The DNA sequence of the urea amidolyase (DUR1,2) gene from S. cerevisiae has been determined. The polypeptide structure deduced from the DNA sequence contains 1,835 amino acid residues and possesses a calculated weight of 201,665 daltons which favorably correlates with that predicted from compositional analysis of purified protein (1,881 amino acid residues and a molecular weight of 203,900). The C-terminal 57 residues of the polypeptide exhibit significant homology with similarly situated sequences found in five other biotin carboxylases whose primary structures have been determined or deduced from protein and DNA sequence data, respectively. Major S1 nuclease protection fragments derived from DUR1,2 RNA-DNA hybrids exhibit apparent termini at positions - 140 and - 141 upstream of the coding region. The termini of minor protection fragments also occur at eleven other positions as well.

Original languageEnglish (US)
Pages (from-to)19-32
Number of pages14
JournalMitochondrial DNA
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1991

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Saccharomyces cerevisiae
biotin carboxylase
Genes
Amino Acids
Peptides
Proteins
Molecular Weight
RNA
Weights and Measures
DNA
urea carboxylase (hydrolyzing)

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

The urea amidolyase (DUR1,2) gene of saccharomyces cerevisiae. / Genbauffe, Francis S.; Cooper, Terrance.

In: Mitochondrial DNA, Vol. 2, No. 1, 01.01.1991, p. 19-32.

Research output: Contribution to journalArticle

Genbauffe, Francis S. ; Cooper, Terrance. / The urea amidolyase (DUR1,2) gene of saccharomyces cerevisiae. In: Mitochondrial DNA. 1991 ; Vol. 2, No. 1. pp. 19-32.
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