The urine microRNA profile may help monitor post-transplant renal graft function

Daniel Maluf, Catherine I. Dumur, Jihee L. Suh, Mariano J. Scian, Anne L. King, Helen Cathro, Jae K. Lee, Ricardo C. Gehrau, Kenneth L. Brayman, Lorenzo Gallon, Valeria Mas

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Noninvasive, cost-effective biomarkers that allow accurate monitoring of graft function are needed in kidney transplantation. Since microRNAs (miRNAs) have emerged as promising disease biomarkers, we sought to establish an miRNA signature in urinary cell pellets comparing kidney transplant patients diagnosed with chronic allograft dysfunction (CAD) with interstitial fibrosis and tubular atrophy and those recipients with normal graft function. Overall, we evaluated 191 samples from 125 deceased donor primary kidney transplant recipients in the discovery, initial validation, and the longitudinal validation studies for noninvasive monitoring of graft function. Of 1733 mature miRNAs studied using microarrays, 22 were found to be differentially expressed between groups. Ontology and pathway analyses showed inflammation as the principal biological function associated with these miRNAs. Twelve selected miRNAs were longitudinally evaluated in urine samples of an independent set of 66 patients, at two time points after kidney transplant. A subset of these miRNAs was found to be differentially expressed between groups early after kidney transplant before histological allograft injury was evident. Thus, a panel of urine miRNAs was identified as potential biomarkers for monitoring graft function and anticipating progression to CAD in kidney transplant patients.

Original languageEnglish (US)
Pages (from-to)439-449
Number of pages11
JournalKidney International
Volume85
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

MicroRNAs
Urine
Transplants
Kidney
Allografts
Biomarkers
Validation Studies
Kidney Transplantation
Atrophy
Longitudinal Studies
Fibrosis
Tissue Donors
Inflammation
Costs and Cost Analysis
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

The urine microRNA profile may help monitor post-transplant renal graft function. / Maluf, Daniel; Dumur, Catherine I.; Suh, Jihee L.; Scian, Mariano J.; King, Anne L.; Cathro, Helen; Lee, Jae K.; Gehrau, Ricardo C.; Brayman, Kenneth L.; Gallon, Lorenzo; Mas, Valeria.

In: Kidney International, Vol. 85, No. 2, 01.01.2014, p. 439-449.

Research output: Contribution to journalArticle

Maluf, D, Dumur, CI, Suh, JL, Scian, MJ, King, AL, Cathro, H, Lee, JK, Gehrau, RC, Brayman, KL, Gallon, L & Mas, V 2014, 'The urine microRNA profile may help monitor post-transplant renal graft function', Kidney International, vol. 85, no. 2, pp. 439-449. https://doi.org/10.1038/ki.2013.338
Maluf, Daniel ; Dumur, Catherine I. ; Suh, Jihee L. ; Scian, Mariano J. ; King, Anne L. ; Cathro, Helen ; Lee, Jae K. ; Gehrau, Ricardo C. ; Brayman, Kenneth L. ; Gallon, Lorenzo ; Mas, Valeria. / The urine microRNA profile may help monitor post-transplant renal graft function. In: Kidney International. 2014 ; Vol. 85, No. 2. pp. 439-449.
@article{af165318ecaa450ba98268dc87f46785,
title = "The urine microRNA profile may help monitor post-transplant renal graft function",
abstract = "Noninvasive, cost-effective biomarkers that allow accurate monitoring of graft function are needed in kidney transplantation. Since microRNAs (miRNAs) have emerged as promising disease biomarkers, we sought to establish an miRNA signature in urinary cell pellets comparing kidney transplant patients diagnosed with chronic allograft dysfunction (CAD) with interstitial fibrosis and tubular atrophy and those recipients with normal graft function. Overall, we evaluated 191 samples from 125 deceased donor primary kidney transplant recipients in the discovery, initial validation, and the longitudinal validation studies for noninvasive monitoring of graft function. Of 1733 mature miRNAs studied using microarrays, 22 were found to be differentially expressed between groups. Ontology and pathway analyses showed inflammation as the principal biological function associated with these miRNAs. Twelve selected miRNAs were longitudinally evaluated in urine samples of an independent set of 66 patients, at two time points after kidney transplant. A subset of these miRNAs was found to be differentially expressed between groups early after kidney transplant before histological allograft injury was evident. Thus, a panel of urine miRNAs was identified as potential biomarkers for monitoring graft function and anticipating progression to CAD in kidney transplant patients.",
author = "Daniel Maluf and Dumur, {Catherine I.} and Suh, {Jihee L.} and Scian, {Mariano J.} and King, {Anne L.} and Helen Cathro and Lee, {Jae K.} and Gehrau, {Ricardo C.} and Brayman, {Kenneth L.} and Lorenzo Gallon and Valeria Mas",
year = "2014",
month = "1",
day = "1",
doi = "10.1038/ki.2013.338",
language = "English (US)",
volume = "85",
pages = "439--449",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - The urine microRNA profile may help monitor post-transplant renal graft function

AU - Maluf, Daniel

AU - Dumur, Catherine I.

AU - Suh, Jihee L.

AU - Scian, Mariano J.

AU - King, Anne L.

AU - Cathro, Helen

AU - Lee, Jae K.

AU - Gehrau, Ricardo C.

AU - Brayman, Kenneth L.

AU - Gallon, Lorenzo

AU - Mas, Valeria

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Noninvasive, cost-effective biomarkers that allow accurate monitoring of graft function are needed in kidney transplantation. Since microRNAs (miRNAs) have emerged as promising disease biomarkers, we sought to establish an miRNA signature in urinary cell pellets comparing kidney transplant patients diagnosed with chronic allograft dysfunction (CAD) with interstitial fibrosis and tubular atrophy and those recipients with normal graft function. Overall, we evaluated 191 samples from 125 deceased donor primary kidney transplant recipients in the discovery, initial validation, and the longitudinal validation studies for noninvasive monitoring of graft function. Of 1733 mature miRNAs studied using microarrays, 22 were found to be differentially expressed between groups. Ontology and pathway analyses showed inflammation as the principal biological function associated with these miRNAs. Twelve selected miRNAs were longitudinally evaluated in urine samples of an independent set of 66 patients, at two time points after kidney transplant. A subset of these miRNAs was found to be differentially expressed between groups early after kidney transplant before histological allograft injury was evident. Thus, a panel of urine miRNAs was identified as potential biomarkers for monitoring graft function and anticipating progression to CAD in kidney transplant patients.

AB - Noninvasive, cost-effective biomarkers that allow accurate monitoring of graft function are needed in kidney transplantation. Since microRNAs (miRNAs) have emerged as promising disease biomarkers, we sought to establish an miRNA signature in urinary cell pellets comparing kidney transplant patients diagnosed with chronic allograft dysfunction (CAD) with interstitial fibrosis and tubular atrophy and those recipients with normal graft function. Overall, we evaluated 191 samples from 125 deceased donor primary kidney transplant recipients in the discovery, initial validation, and the longitudinal validation studies for noninvasive monitoring of graft function. Of 1733 mature miRNAs studied using microarrays, 22 were found to be differentially expressed between groups. Ontology and pathway analyses showed inflammation as the principal biological function associated with these miRNAs. Twelve selected miRNAs were longitudinally evaluated in urine samples of an independent set of 66 patients, at two time points after kidney transplant. A subset of these miRNAs was found to be differentially expressed between groups early after kidney transplant before histological allograft injury was evident. Thus, a panel of urine miRNAs was identified as potential biomarkers for monitoring graft function and anticipating progression to CAD in kidney transplant patients.

UR - http://www.scopus.com/inward/record.url?scp=84895902220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84895902220&partnerID=8YFLogxK

U2 - 10.1038/ki.2013.338

DO - 10.1038/ki.2013.338

M3 - Article

VL - 85

SP - 439

EP - 449

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 2

ER -