The viable but nonculturable state induction and genomic analyses of Lactobacillus casei BM-LC14617, a beer-spoilage bacterium

Junyan Liu, Lin Li, Brian Peters, Bing Li, Lequn Chen, Yang Deng, Zhenbo Xu, Mark E. Shirtliff

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study aimed to investigate the viable but nonculturable (VBNC) state and genomic features of a beer-spoilage strain, Lactobacillus caseiBM-LC14617. Induction on the VBNC state of L. casei strain BM-LC14617 was conducted by both low-temperature storage and continuous passage in beer, and formation of VBNC state was detected after 196 ± 3.3 days and 32 ± 1.6 subcultures, respectively. Resuscitation of VBNC cells was successfully induced by addition of catalase, and culturable, VBNC, and resuscitated cells shared similar beer-spoilage capability. Whole genome sequencing was performed, and out of a total of 3,964 predicted genes, several potential VBNC and beer-spoilage-associated genes were identified. L. casei is capable of entering into and resuscitating from the VBNC state and possesses beer-spoilage capability. The genomic characterization yield insightful elucidation of VBNC state for L. casei. This study represents the first evidence on VBNC state induction of L. casei and beer-spoilage capability of VBNC and resuscitated cells. Also, this is the first genomic characterization of L. casei as a beer-spoilage bacterium. The current study may aid in further study on L. casei and other beer-spoilage bacteria, and guide the prevention and control of beer spoilage.

Original languageEnglish (US)
Article numbere00506
JournalMicrobiologyOpen
Volume6
Issue number5
DOIs
StatePublished - Oct 1 2017

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Lactobacillus casei
Bacteria
Lactobacillus
Resuscitation
Catalase
Genes
Genome
Temperature

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

The viable but nonculturable state induction and genomic analyses of Lactobacillus casei BM-LC14617, a beer-spoilage bacterium. / Liu, Junyan; Li, Lin; Peters, Brian; Li, Bing; Chen, Lequn; Deng, Yang; Xu, Zhenbo; Shirtliff, Mark E.

In: MicrobiologyOpen, Vol. 6, No. 5, e00506, 01.10.2017.

Research output: Contribution to journalArticle

Liu, Junyan ; Li, Lin ; Peters, Brian ; Li, Bing ; Chen, Lequn ; Deng, Yang ; Xu, Zhenbo ; Shirtliff, Mark E. / The viable but nonculturable state induction and genomic analyses of Lactobacillus casei BM-LC14617, a beer-spoilage bacterium. In: MicrobiologyOpen. 2017 ; Vol. 6, No. 5.
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