The yeast ADP/ATP carrier. Mutagenesis and second-site revertants

Research output: Contribution to journalArticle

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Abstract

Results of mutagenesis and selection of spontaneous second-site revertants of the yeast ADP/ATP carrier AAC2 is described. Currently, 50 mutants have been made in AAC2 at 35 locations. Yeast carrying mutations at K38, K48, R96, D149, R152, R204, D249, R252, R253, R254 and R294 are all unable to grow on glycerol. Seven of these mutants have yielded second-site revertants when plated on rich yeast media containing glycerol and ethanol. The R96 mutants and the R254 and R253 mutants produce similar changes in the AAC2 molecule because the same sites are affected by their revertant mutations. This system of mutations and revertants is now poised to yield insights into the dynamics of ADP and ATP transport, and mitochondrial carrier structure in general.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1275
Issue number1-2
DOIs
StatePublished - Jul 18 1996

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ATP Translocases Mitochondrial ADP
Mutagenesis
Yeast
Yeasts
Glycerol
Mutation
Adenosine Diphosphate
Ethanol
Adenosine Triphosphate
Molecules

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

The yeast ADP/ATP carrier. Mutagenesis and second-site revertants. / Nelson, David.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1275, No. 1-2, 18.07.1996, p. 133-137.

Research output: Contribution to journalArticle

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