Thermoresponsive terpolymeric films applicable for osteoblastic cell growth and noninvasive cell sheet harvesting

Young Shin Kim, Jung Yul Lim, Henry J. Donahue, Tao Lowe

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A novel multifunctional linear block copolymer, poly(N-isopropylacrylamide- co-acrylic acid)-b-poly(L-lactic acid) (NAL), was synthesized to expand the concept of cell sheet engineering by using its thermoresponsive property and processibility. The chemical structure of synthesized NAL was confirmed by Fourier transform infrared spectroscopy, and its molar mass (103,500 g · mol-1) and molar mass distribution were determined by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy. NAL copolymer was fabricated into thin films by spin-casting. Spin-cast NAL films displayed thermoresponsive properties as demonstrated by surface wettability and topology changes from relatively more hydrophobic (contact angle of 56°) and rougher at 37°C to relatively more hydrophilic (contact angle of 40°) and smoother at 22°C, as assessed by contact angle measurement and atomic force microscopy, respectively. Murine osteoblastic MC3T3-E1 cells displayed comparable adhesion but slower proliferation on NAL films than on poly(L-lactic acid) (PLLA) films and tissue culture polystyrene (TCPS). Within 9 days of cell culture, the highest alkaline phosphatase activity of MC3T3-E1 cells occurred later (on day 9) on NAL films than on PLLA films and TCPS (on day 6). A well-established MC3T3-E1 cell sheet was successfully detached from NAL films, in the absence of enzymes, within about 5 min by simply lowering the temperature from 37°C to room temperature. NAL copolymer has potential for use in the controlled release of therapeutic agents while simultaneously supporting cell growth. In addition, it may be applicable for noninvasive two- or three-dimensional cell sheet harvesting.

Original languageEnglish (US)
Pages (from-to)30-40
Number of pages11
JournalTissue Engineering
Volume11
Issue number1-2
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

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Cell growth
Lactic acid
Contact angle
Tissue culture
Molar mass
Polystyrenes
Copolymers
Phosphatases
Angle measurement
Cell culture
Block copolymers
Acrylics
Ionization
Fourier transform infrared spectroscopy
Wetting
Atomic force microscopy
Desorption
Casting
Adhesion
Enzymes

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Thermoresponsive terpolymeric films applicable for osteoblastic cell growth and noninvasive cell sheet harvesting. / Kim, Young Shin; Lim, Jung Yul; Donahue, Henry J.; Lowe, Tao.

In: Tissue Engineering, Vol. 11, No. 1-2, 01.01.2005, p. 30-40.

Research output: Contribution to journalArticle

Kim, Young Shin ; Lim, Jung Yul ; Donahue, Henry J. ; Lowe, Tao. / Thermoresponsive terpolymeric films applicable for osteoblastic cell growth and noninvasive cell sheet harvesting. In: Tissue Engineering. 2005 ; Vol. 11, No. 1-2. pp. 30-40.
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