Thioredoxin is related to life span regulation and oxidative stress response in Caenorhabditis elegans

Chang Hoon Jee, Liviu Vanoaica, Jungsoo Lee, Byung Jae Park, Joohong Ahnn

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Thioredoxin, an oxidoreductase, is a multifunction protein. The thioredoxin system is composed of NADPH, thioredoxin reductase and thioredoxin. This enzyme is highly conserved from bacteria to humans. We have characterized TRX-1, a thioredoxin homolog in C. elegans, which has about 36% identity in amino acid sequence with human thioredoxin. By gfp reporter system, trx-1 has been shown to be restrictedly expressed in ASI and ASJ neurons and in intestine. Immunostaining confirmed the intestinal expression. Full-length cDNA of trx-1 has been isolated by cDNA library PCR and subsequently cloned and sequenced. We have shown that the encoded protein functions as a reductase in the insulin reducing assay. Moreover, we have isolated a deletion mutant by PCR-based TMP-UV mutagenesis method. Mutant animals have reduced life span and are sensitive to oxidative stress. Reintroduction of trx-1 into mutant worms fully restored the wild-type phenotype. Our results suggest that trx-1 has important functions in life span regulation and oxidative stress response in C. elegans.

Original languageEnglish (US)
Pages (from-to)1203-1210
Number of pages8
JournalGenes to Cells
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2005

Fingerprint

Thioredoxins
Caenorhabditis elegans
Oxidative Stress
Oxidoreductases
Thymidine Monophosphate
Thioredoxin-Disulfide Reductase
Polymerase Chain Reaction
Gene Library
Mutagenesis
Intestines
Amino Acid Sequence
Proteins
Complementary DNA
Insulin
Bacteria
Phenotype
Neurons
Enzymes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Thioredoxin is related to life span regulation and oxidative stress response in Caenorhabditis elegans. / Jee, Chang Hoon; Vanoaica, Liviu; Lee, Jungsoo; Park, Byung Jae; Ahnn, Joohong.

In: Genes to Cells, Vol. 10, No. 12, 01.12.2005, p. 1203-1210.

Research output: Contribution to journalArticle

Jee, Chang Hoon ; Vanoaica, Liviu ; Lee, Jungsoo ; Park, Byung Jae ; Ahnn, Joohong. / Thioredoxin is related to life span regulation and oxidative stress response in Caenorhabditis elegans. In: Genes to Cells. 2005 ; Vol. 10, No. 12. pp. 1203-1210.
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