Three prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth

Douglas A. Johnston, Arturo Luna Tapia, Karen E. Eberle, Glen Palmer

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Disruption of vacuolar biogenesis in the pathogenic yeast Candida albicans causes profound defects in polarized hyphal growth. However, the precise vacuolar pathways involved in yeast-hypha differentiation have not been determined. Previously we focused on Vps21p, a Rab GTPase involved in directing vacuolar trafficking through the late endosomal prevacuolar compartment (PVC). Herein, we identify two additional Vps21p-related GTPases, Ypt52p and Ypt53p, that colocalize with Vps21p and can suppress the hyphal defects of the vps21Δ/Δ mutant. Phenotypic analysis of gene deletion strains revealed that loss of both VPS21 and YPT52 causes synthetic defects in endocytic trafficking to the vacuole, as well as delivery of the virulence-associated vacuolar membrane protein Mlt1p from the Golgi compartment. Transcription of all three GTPase-encoding genes is increased under hyphal growth conditions, and overexpression of the transcription factor Ume6p is sufficient to increase the transcription of these genes. While only the vps21 Δ/Δ single mutant has hyphal growth defects, these were greatly exacerbated in a vps21 Δ/Δ ypt52 Δ/Δ double mutant. On the basis of relative expression levels and phenotypic analysis of gene deletion strains, Vps21p is the most important of the three GTPases, followed by Ypt52p, while Ypt53p has an only marginal impact on C. albicans physiology. Finally, disruption of a nonendosomal AP-3-dependent vacuolar trafficking pathway in the vps21 Δ/Δ ypt52 Δ/Δ mutant, further exacerbated the stress and hyphal growth defects. These findings underscore the importance of membrane trafficking through the PVC in sustaining the invasive hyphal growth form of C. albicans.

Original languageEnglish (US)
Pages (from-to)1039-1050
Number of pages12
JournalEukaryotic Cell
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2013

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rab GTP-Binding Proteins
Candida albicans
GTP Phosphohydrolases
Growth
Gene Deletion
Yeasts
Hyphae
Vacuoles
Genes
Virulence
Membrane Proteins
Transcription Factors
Membranes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Three prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth. / Johnston, Douglas A.; Luna Tapia, Arturo; Eberle, Karen E.; Palmer, Glen.

In: Eukaryotic Cell, Vol. 12, No. 7, 01.07.2013, p. 1039-1050.

Research output: Contribution to journalArticle

Johnston, Douglas A. ; Luna Tapia, Arturo ; Eberle, Karen E. ; Palmer, Glen. / Three prevacuolar compartment Rab GTPases impact Candida albicans hyphal growth. In: Eukaryotic Cell. 2013 ; Vol. 12, No. 7. pp. 1039-1050.
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