Time course study on the development of allergen-induced airway remodeling in mice: The effect of allergen avoidance on established airway remodeling

M. B. Snijder, M. Visser, J. M. Dekker, J. C. Seidell, T. Fuerst, Frances Tylavsky, J. Cauley, T. Lang, M. Nevitt, T. B. Harris

Research output: Contribution to journalArticle

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Abstract

Objective and design: We carried out a time course study on the development of allergen-induced airway remodeling in a mouse model of allergic asthma. Moreover, we examined the effect of allergen avoidance on the established airway remodeling. Materials and methods: BALB/c mice were sensitized to ovalbumin (OA) with alum, and exposed daily for 3 weeks to aerosolized OA. At each designated point, bronchial responsiveness was measured, and bronchoalveolar lavage and histological examination were carried out. Results: The numbers of inflammatory leukocytes in the airways and the percentage of goblet cells in the epithelium, Th2 cytokine production, IgE production, collagen deposition beneath the basement membrane and bronchial responsiveness to acetylcholine were all markedly increased after repeated antigen challenge for 1-3 weeks. In contrast, after cessation of antigen exposure, goblet cell hyperplasia, inflammatory infiltrates and bronchial hyperresponsiveness were gradually attenuated and had almost resolved 4 weeks after cessation, but subepithelial fibrosis was still observed at this time point. Conclusions: The present findings demonstrated that epithelial changes following repeated allergen challenge are rapidly induced and recover after the cessation of exposure, but subepithelial fibrosis has a late onset and relatively irreversible changes, and subepithelial fibrosis in contrast to goblet cells hyperplasia did not appear to contribute to bronchial hyperresponsiveness, at least, in this mouse model.

Original languageEnglish (US)
Pages (from-to)307-316
Number of pages10
JournalInflammation Research
Volume51
Issue number6
StatePublished - Jan 1 2002

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Airway Remodeling
Goblet Cells
Allergens
Fibrosis
Hyperplasia
Antigens
Ovalbumin
Bronchoalveolar Lavage
Leukocyte Count
Basement Membrane
Immunoglobulin E
Acetylcholine
Collagen
Asthma
Epithelium
Cytokines

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

Cite this

Time course study on the development of allergen-induced airway remodeling in mice : The effect of allergen avoidance on established airway remodeling. / Snijder, M. B.; Visser, M.; Dekker, J. M.; Seidell, J. C.; Fuerst, T.; Tylavsky, Frances; Cauley, J.; Lang, T.; Nevitt, M.; Harris, T. B.

In: Inflammation Research, Vol. 51, No. 6, 01.01.2002, p. 307-316.

Research output: Contribution to journalArticle

Snijder, MB, Visser, M, Dekker, JM, Seidell, JC, Fuerst, T, Tylavsky, F, Cauley, J, Lang, T, Nevitt, M & Harris, TB 2002, 'Time course study on the development of allergen-induced airway remodeling in mice: The effect of allergen avoidance on established airway remodeling', Inflammation Research, vol. 51, no. 6, pp. 307-316.
Snijder, M. B. ; Visser, M. ; Dekker, J. M. ; Seidell, J. C. ; Fuerst, T. ; Tylavsky, Frances ; Cauley, J. ; Lang, T. ; Nevitt, M. ; Harris, T. B. / Time course study on the development of allergen-induced airway remodeling in mice : The effect of allergen avoidance on established airway remodeling. In: Inflammation Research. 2002 ; Vol. 51, No. 6. pp. 307-316.
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