Titanium particles and surface-bound LPS activate different pathways in IC-21 macrophages

Luciana P. Schwab, Zhiqing Xing, Karen Hasty, Richard Smith

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

It is still unknown if wear-debris particles themselves induce osteolysis or if they serve a functional role as receptors for ligands that incite an inflammatory response that ultimately leads to bone resorption. In this study, commercially pure titanium particles (cpTi) were subjected to a serial combination of different cleaning methods to remove Lipopolysaccharide (LPS) or were incubated in LPS solutions of known concentrations. Then, the response of the macrophage cell line IC-21 to the cleaned particles, LPS-bound Ti particles, and soluble LPS was examined. It was found that cleaned particles up to 1000 particles per cell did not stimulate macrophages to release Tumor necrosis factor-alpha (TNF-α) or Interleukin 6 (IL-6), but they significantly increased the release of Prostaglandin E2 (PGE2) when the particle concentration was higher than 500 particles per cell. At one particle per cell, Ti particles bound with LPS stimulated the release of IL-6 and TNF-α by macrophages. The level of released cytokines was dependent on, and correlated with, the amount of LPS present on the particles. The macrophages were more sensitive to soluble LPS than to particle-bound LPS, and the simultaneous addition of cleaned Ti particles did not have additional effects on the effects of soluble LPS. This study shows evidence that, cpTi particles and LPS have distinct mechanisms of action on the IC-21 macrophages, but that both contribute to the development of an inflammatory response.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume79
Issue number1
DOIs
StatePublished - Oct 1 2006

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Macrophages
Titanium
Lipopolysaccharides
Debris
Cleaning
Interleukin-6
Bone
Tumor Necrosis Factor-alpha
Ligands
Cells
Wear of materials
Dinoprostone
Cytokines

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Titanium particles and surface-bound LPS activate different pathways in IC-21 macrophages. / Schwab, Luciana P.; Xing, Zhiqing; Hasty, Karen; Smith, Richard.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 79, No. 1, 01.10.2006, p. 66-73.

Research output: Contribution to journalArticle

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