Topography of a flexible ribonucleoprotein helix

Protein-protein contacts in Sendai virus nucleocapsids

Rajendra Raghow, D. W. Kingsbury, A. Portner, S. George

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Contacts among the three polypeptide species in the flexible helical nucleocapsids of a paramyxovirus were examined with bifunctional protein cross-linking reagents. Polypeptides L and P, minor components of Sendai virus nucleocapsids implicated in viral RNA polymerase activity, were efficiently cross-linked into large complexes, indicating that they enjoy abundant contacts with neigboring protein molecules in the helix. Less reactivity was found in the case of the major structural polypeptide, NP; about half of all molecules of NP formed large cross-linked complexes, most of the rest remaining as monomers along with a small proportion of homodimers and low-order oligomers. Marked heterogeneity in the cross-linking reactivity of NP molecules, which may reflect the conformational quasi-equivalence inherent in a flexible helix, was indicated by the production of several conformers of homodimers and other low-order oligomers of NP, and by failure of the kinetics of NP cross-linking to conform to a simple statistical model of random polymerization. The validity of the statistical model was shown by cross-linking experiments with the rigid helical virus, tobacco mosaic virus.

Original languageEnglish (US)
Pages (from-to)701-710
Number of pages10
JournalJournal of Virology
Volume30
Issue number3
StatePublished - Dec 1 1979
Externally publishedYes

Fingerprint

Sendai virus
Nucleocapsid
ribonucleoproteins
nucleocapsid
Ribonucleoproteins
Statistical Models
crosslinking
topography
polypeptides
Cross-Linking Reagents
statistical models
Tobacco Mosaic Virus
Peptides
Viral RNA
DNA-Directed RNA Polymerases
Polymerization
Respirovirus
Proteins
Tobacco mosaic virus
proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Topography of a flexible ribonucleoprotein helix : Protein-protein contacts in Sendai virus nucleocapsids. / Raghow, Rajendra; Kingsbury, D. W.; Portner, A.; George, S.

In: Journal of Virology, Vol. 30, No. 3, 01.12.1979, p. 701-710.

Research output: Contribution to journalArticle

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